- A phenolic amide and other constituents of Melandrium firmum
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α-Spinasterylglucoside, linarin and a new phenolic acid amide named melandrin were isolated from Melandrium firmum (Caryophyllaceae). The structure was elucidated as N-p-hydroxybenzoyl-5-hydroxyanthranilic acid on the basis of its spectral data and synthesis.
- Woo, Won Sick,Choi, Jae Sue
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- Induction of hydroxyanthranilate hydroxycinnamoyl transferase activity by oligo-N-acetylchitooligosaccharides in oats
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An assay method for hydroxycinnamoyl-CoA: hydroxyanthranilate N- hydroxycinnamoyl transferase (HHT) in oat leaves (Avena sativa L.), which is thought to be one of the key enzymes for the biosynthesis of avenanthramides, phytoalexins in this plant, was established. HHT activity was induced by treating the leaves with oligo-N-acetylchitooligosaccharides. Among the chitooligosaccharides tested, penta-N-acetylchitopentaose ((GlcNAc)5) was the most effective in inducing activity. The induction by (GlcNAc)5 was dose-dependent, in which case HHT activity was initially detected after 6 hr and reached a maximum by 12 hr. All of the putative precursors of avenanthramides acted as substrates for HHT, with 5-hydroxyanthranilic acid and feruloyl-CoA being the best substrates for the anthranilic moiety and the cinnamoyl moiety of avenanthramides, respectively.
- Ishihara, Atsushi,Miyagawa, Hisashi,Matsukawa, Tetsuya,Ueno, Tamio,Mayama, Shigeyuki,Iwamura, Hajime
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