- ROLE OF AMINO ACID RESIDUES IN CHROMOGENIC SUBSTRATES CLEAVED BY PANCREATIC ELASTASE
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Anionic chromogenic substrates, 3-carboxypropionyl tripeptide p-nitroanilides modified with glycine, β-alanine, alanine, leucine, and proline in positions P1, P2, and P3 were synthesized.The substrates were digested with pancreatic elastase and the values of Km, kcat, and kcat/Km were determined.Alanine plays a decisive role in position P1, substrates with glycine or β-alanine in this position are not cleaved.The substitution in P2 is dominant for proline; next follow alanine, leucine, and glycine.The substitution in P3 is the least specific one.
- Kasafirek, Evzen,Fric, Premysl,Slaby, Jan
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p. 1625 - 1633
(2007/10/02)
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