Tetramethylrhodamine-5-iodoacetamidedihydroiodide (5-TMRIA) is a fluorescent dye belonging to the rhodamine family, known for its bright red fluorescence. It is a chemical compound widely utilized in bioconjugation and fluorescent labeling due to its high photostability and resistance to photobleaching, making it an ideal choice for the labeling and visualization of biomolecules such as proteins and antibodies in various research fields.
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Basic information
- Product Name: Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)
- Synonyms: 9-[2-Carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)xanthylium inner salt;Tetramethylrhodamine-4-iodoacetamide;Tetramethylrhodamine-5-iodoacetamide dihydroiodide;5-TMRIA [TetraMethylrhodaMine-5-IodoacetaMide Dihydroiodide];VINNHWDBTRUVKY-UHFFFAOYSA-N
- CAS NO:114458-99-0
- Molecular Formula: C26H24IN3O4
- Molecular Weight: 569.39093
- EINECS: N/A
- Product Categories: N/A
- Mol File: 114458-99-0.mol
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: N/A
- Solubility: N/A
- CAS DataBase Reference: Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)(CAS DataBase Reference)
- NIST Chemistry Reference: Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)(114458-99-0)
- EPA Substance Registry System: Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)(114458-99-0)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany:
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 114458-99-0(Hazardous Substances Data)
114458-99-0 Usage
Uses
Used in Research Fields:Tetramethylrhodamine-5-iodoacetamidedihydroiodide (5-TMRIA) is used as a fluorescent label for biomolecules in research fields such as immunology, cell biology, and molecular biology. It aids in the visualization and analysis of these biomolecules due to its high photostability and resistance to photobleaching.
Used in Bioconjugation:
5-TMRIA is used as a bioconjugation agent for the creation of fluorescent bioconjugates with thiol-containing proteins. This allows for the development of various analytical and diagnostic techniques that require the labeling of biomolecules.
Used in Analytical and Diagnostic Techniques:
In Immunofluorescence Microscopy:
Tetramethylrhodamine-5-iodoacetamidedihydroiodide (5-TMRIA) is used as a fluorescent probe for the detection and localization of specific proteins or antibodies within cells or tissues, providing high-resolution imaging.
In Flow Cytometry:
5-TMRIA is used as a fluorescent marker for the identification and quantification of specific cell populations based on the presence of labeled antibodies, enabling rapid and accurate cell analysis.
In Fluorescent In Situ Hybridization (FISH):
Tetramethylrhodamine-5-iodoacetamidedihydroiodide (5-TMRIA) is used as a fluorescent tag for the visualization of specific DNA or RNA sequences within cells, allowing for the study of gene expression and chromosomal abnormalities.
Check Digit Verification of cas no
The CAS Registry Mumber 114458-99-0 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,4,4,5 and 8 respectively; the second part has 2 digits, 9 and 9 respectively.Calculate Digit Verification of CAS Registry Number 114458-99:
(8*1)+(7*1)+(6*4)+(5*4)+(4*5)+(3*8)+(2*9)+(1*9)=130
130 % 10 = 0
So 114458-99-0 is a valid CAS Registry Number.
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Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)
