High rates and substrate selectivities in water by polyvinylimidazoles as transaminase enzyme mimics with hydrophobically bound pyridoxamine derivatives as coenzyme mimics
(Chemical Equation Presented) Free-radical polymers of 4-vinylimidazole and copolymers with 1-dodecyl-4-vinylimidazole were used as enzyme mimics to transaminate pyruvic acid to alanine, phenylpyruvic acid to phenylalanine, and indole-3-pyruvic acid to tryptophan in water at pH 7.5 and 20 °C using pyridoxamines carrying hydrophobic side chains as coenzyme mimics. The best enzyme mimic accelerated the transamination of indole-3-pyruvic acid by a factor of 4 million relative to the rate without the polymer, a higher rate ratio than we had previously achieved with a polyaziridine-based enzyme mimic. The properties of various polyvinylimidazoles were compared, including those prepared with the RAFT modification of the polymerization process.
Skouta, Rachid,Wei, Sujun,Breslow, Ronald
supporting information; experimental part
p. 15604 - 15605
(2010/01/30)
Universal (Switchable) RAFT agents
-
Benaglia, Massimo,Chiefari, John,Chong, Yen K.,Moad, Graeme,Rizzardo, Ezio,Thang, San H.
supporting information; experimental part
p. 6914 - 6915
(2009/09/26)
More Articles about upstream products of 1158958-94-1