Studies on the biodegradation of fosfomycin: Synthesis of 13C-labeled intermediates, feeding experiments with rhizobium huakuii PMY1, and Isolation of Labeled Amino Acids from Cell Mass by HPLC
Racemic (1R*,2R*)-1,2-dihydroxy-[1-13C 1]propylphosphonic acid and 1-hydroxy-[1-13C 1]acetone were synthesized and fed to R. huakuii PMY1. Alanine and a mixture of valine and methionine were isolated as their N-acetyl derivatives from the cell hydrolysate by reversed-phase HPLC and analyzed by NMR spectroscopy. It was found that the carbon atoms of the respective carboxyl groups were highly 13C-labeled (up to 65 %). Hydroxyacetone is therefore considered an obligatory intermediate of the biodegradation of fosfomycin by R. huakuii PMY1.
McGrath, John W.,Hammerschmidt, Friedrich,Kaehlig, Hanspeter,Wuggenig, Frank,Lamprecht, Guenther,Quinn, John P.
experimental part
p. 13341 - 13348
(2012/03/22)
Studies on the biodegradation of fosfomycin: Growth of Rhizobium huakuii PMY1 on possible intermediates synthesised chemically
The first step of the mineralisation of fosfomycin by R. huakuii PMY1 is hydrolytic ring opening with the formation of (1R,2R)-1,2- dihydroxypropylphosphonic acid. This phosphonic acid and its three stereoisomers were synthesised by chemical means and tes
McGrath, John W.,Hammerschmidt, Friedrich,Preusser, Werner,Quinn, John P.,Schweifer, Anna
experimental part
p. 1944 - 1953
(2009/06/28)
More Articles about upstream products of 1160525-87-0