125440-91-7 Usage
Uses
Used in Analytical Chemistry:
3-PHENOXAZONE 7-[BETA-D-GLUCURONIDE] SODIUM SALT is used as an analytical reagent for detecting and quantifying enzymatic activities. It serves as a substrate for enzymes such as beta-glucuronidase and glucuronosyltransferases, which are involved in the metabolism and detoxification processes in living organisms. The compound's conversion to resorufin upon enzymatic action allows for the measurement of enzyme activity through fluorescence or colorimetric assays.
Used in Biological Research:
In the field of biological research, 3-PHENOXAZONE 7-[BETA-D-GLUCURONIDE] SODIUM SALT is used as a tool for studying enzyme kinetics, enzyme inhibition, and enzyme mechanisms. The compound's conversion to resorufin provides a sensitive and reliable method for monitoring enzyme activity in various experimental setups, including high-throughput screening assays and single-cell digital analysis using droplet-based microfluidics.
Used in Pharmaceutical Industry:
3-PHENOXAZONE 7-[BETA-D-GLUCURONIDE] SODIUM SALT is used as a testing agent for evaluating the efficacy of drugs targeting enzymes involved in glucuronidation pathways. These pathways are crucial for the metabolism and elimination of various drugs and xenobiotics from the body. By monitoring the activity of glucuronidation enzymes using this compound, researchers can assess the potential drug-drug interactions and the metabolic profile of new drug candidates.
Used in Teaching and Education:
In the context of teaching and education, 3-PHENOXAZONE 7-[BETA-D-GLUCURONIDE] SODIUM SALT is used as a model compound for demonstrating enzyme-catalyzed reactions and the principles of enzyme kinetics. Its use in single-cell digital analysis using droplet-based microfluidics also provides an opportunity for students to learn about advanced analytical techniques and their applications in biological research.
Biochem/physiol Actions
Resorufin β-D-glucuronide serves as a substrate for β-glucuronidase enzyme. It is considered as an internal hydrolysis indicator for enzyme action. Resorufin β-D-glucuronide possesses a high extinction coefficient.
Check Digit Verification of cas no
The CAS Registry Mumber 125440-91-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,5,4,4 and 0 respectively; the second part has 2 digits, 9 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 125440-91:
(8*1)+(7*2)+(6*5)+(5*4)+(4*4)+(3*0)+(2*9)+(1*1)=107
107 % 10 = 7
So 125440-91-7 is a valid CAS Registry Number.
InChI:InChI=1/C18H15NO9.Na/c20-7-1-3-9-11(5-7)27-12-6-8(2-4-10(12)19-9)26-18-15(23)13(21)14(22)16(28-18)17(24)25;/h1-6,13-16,18,21-23H,(H,24,25);
