Efficient access to nonhydrolyzable initiator tRNA based on the synthesis of 3'-azido-3'-deoxyadenosine RNA
Flexibility exercised: Hydrolysis-resistant 3′-aminoacyl-tRNA conjugates that contain a stable amide linkage instead of the natural ester are valuable substrates for biochemical studies of ribosomal processes. In a novel preparation of the stable E. coli initiator tRNA derivative 3′- (Nformylmethionyl) amino-tRNAfMet the key feature is the synthesis of 3′-azido oligoribonucleotides using a new functionalized solid support.
Steger, Jessica,Graber, Dagmar,Moroder, Holger,Geiermann, Anna-Skrollan,Aigner, Michaela,Micura, Ronald