- A chloroacetate based ratiometric fluorescent probe for cysteine detection in biosystems
-
The specific detection of cysteine (Cys) over homocysteine (Hcy), glutathione (GSH) and other amino acids is of great significance for studying its biological functions as well as for the diagnosis of related diseases. Chloroacetyl group was often used as a reaction site for cysteine fluorescent probes for its sensitivity and selectivity. However, high background fluorescence and low stability are common problems encountered by such probes. Here, four chloroacetyl group based fluorescent probes (C1, C2, C3, and H4) was synthesized for a comparative study. We found that the inefficient quenching ability of chloroacetyl group turned into an advantage when connected with a ratiometric fluorophore. With the modification of chloroacetyl group, probe H4 displayed excellent ratiometric property and great selectivity for Cys, the stability was also improved. Additionally, the probe was successfully applied for quantitative detection of Cys in fetal bovine serum and real-time imaging in living HeLa cells with low toxicity.
- Liu, Zhengkun,Wang, Qianqian,Wang, Hao,Su, Wenting,Dong, Shouliang
-
supporting information
(2019/10/08)
-
- NITROGEN AND SULFUR-CONTAINING HETEROCYCLE DERIVATIVES
-
The present invention provides nitrogen and sulfur-containing heterocycle compounds to be used as scaffolds and, in particular, nitrogen and sulfur-containing heterocycle compounds having a thiomorpholine core. The compounds herein described may be useful in treating diseases such as diabetes, obesity, cancer, cardiovascular, Alzheimer's, inflammatory, antidepressant, rheumatoid arthritis, multiple scelerosis, allergic rhinitis, asthma as well as viral and bacterial infections. The compounds herein described may also be useful in treating CNS disorders such as but not limited to Schizophrenia, Alzheimer's disease (AD).
- -
-
Page/Page column 44
(2010/04/06)
-
- Novel calcitonin derivative and salt thereof
-
There is disclosed a calcitonin derivative and its salt, represented by the formula: wherein Acm represents an acetamidomethyl group; Y represents the formula: where A represents a sulfur atom or a methylene group; and n represents an integer of 0 or 1; Ala: alanine, Thr: threonine, Leu: leucine, Ser: serine, Cys: cysteine, Val: valine, Gly: glycine, Lys: lysine, Gln: glutamine, His: histidine, Tyr: tyrosine, Pro: proline, Arg: arginine and Asp: aspar-tic acid.
- -
-
-
- Peptides
-
A novel peptide of the formula: STR1 wherein A is hydrogen, alkyl, aralkyl, alkoxyalkyl, hydroxyalkyl or alkoxy; R is STR2 X is --S-- or --(CH2)n -- where n is 1 or 2 has a weaker TRH releasing activity than TRH and a stronger anaesthesia-antagonistic activity, spontaneous movement-stimulant activity or dopamine-potentiating activity than TRH.
- -
-
-