- Method for preparing 5-hydroxytryptamine hydrochloride by utilizing enzymatic fermentation liquor containing 5-hydroxytryptamine
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The invention discloses a method for preparing 5-hydroxytryptamine hydrochloride from 5-hydroxytryptamine-containing enzymatic fermentation liquor, and relates to the technical field of 5-hydroxytryptamine extraction. The method comprises the following steps: concentrating 5-hydroxytryptamine-containing enzymatic fermentation liquor under reduced pressure, mixing the concentrated 5-hydroxytryptamine-containing enzymatic fermentation liquor with an organic solvent, sequentially dropwise adding triethylamine and BOC anhydride at low temperature, heating to 20-25 DEG C after dropwise adding is finished, reacting to obtain an N-BOC-5-hydroxytryptamine reaction solution, adding ethyl acetate, and extracting to obtain an ethyl acetate organic phase; adding ethyl acetate containing hydrochloric acid into the ethyl acetate organic phase for reaction, and performing suction filtration after the reaction is finished to obtain a 5-hydroxytryptamine hydrochloride crude product; and further purifying the obtained 5-hydroxytryptamine hydrochloride crude product to obtain 5-hydroxytryptamine hydrochloride. According t o the invention, high-purity 5-hydroxytryptamine hydrochloride is prepared by optimizing the method for purifying 5-hydroxytryptamine in an enzymatic fermentation liquor, so that the current situation that 5-hydroxytryptamine hydrochloride is difficult to prepare by simply extracting serotonin in the enzymatic fermentation liquor is changed.
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Paragraph 0032; 0035-0037; 0038; 0041-0043; 0044; 0047-0049
(2021/02/13)
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- Preparation method of 5-hydroxytryptamine hydrochloride
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The invention discloses a preparation method of 5-hydroxytryptamine hydrochloride, and particularly relates to a method for efficiently synthesizing the 5-hydroxytryptamine hydrochloride by taking 5-hydroxyindole and nitroethylene as raw materials through addition reaction and reduction reaction. The preparation method of the 5-hydroxytryptamine hydrochloride provided by the invention is a preparation method which is high in yield, low in cost, less in three wastes, good in product purity and suitable for industrialization.
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Paragraph 0029
(2021/02/10)
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