Fluorogenic Substrates Containing 7-Amino-4-methyl-2-quinolinone for Aminopeptidase M, Chymotrypsin, Elastase and Trypsin, Determination of Enzyme Activity
Several new fluorogenic substrates for four proteases were synthesized by coupling the highly fluorescent 7-amino-4-methyl-2-quinolinone (AMeq) with appropriate amino acids and peptides.Compounds HCl*H-Ala-NH-Meq (1) and HCl*H-Leu-NH-Meq (2) are substrates for aminopeptidase M, Glt-Tyr-NH-Meq (3) and Glt-Leu-Tyr-NH-Meq (4) for chymotrypsin, Boc-(Ala)4-NH-Meq (5) for elastase and Z-Arg-NH-Meq (6) for trypsin.The kinetic parameters for the hydrolysis of the new substrates were determined and showed that each new substrate is suitable for a convenient fluorometric assay of the corresponding enzyme. - Key Words: 2-Quinolinone, 7-amino-4-methyl- / Fluorogenic substrates / Aminopeptidase M / Chymotrypsin / Elastase / Trypsin / Enzymes / Amino acids
Tzougraki, Chryssa,Noula, Catherine,Geiger, Reinhard,Kokotos, George
p. 365 - 368
(2007/10/02)
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