- The β oxidative cleavage of long chain fatty acids in rat liver cytoplasm
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A β oxidative cleavage of oleyl and stearyl CoA has been obtained with soluble enzymes of rat liver homogenate. The transformation requires NAD+ as an oxido reductive cofactor and is enhanced by ATP and CoA. Under the reported conditions 2,9 octadecadienoic, 3,9 octadecadienoic, 3 hydroxy 9 octadecenoic, 7 hexadecenoic, 3 hydroxy 7 hexadecenoic and 5 tetradecenoic acids are present after incubation of oleyl CoA, while no detectable amounts of shorter fatty acids are formed. A similar oxidation pattern is obtained with stearyl CoA when Mg2+ ions are also added to the system. The cytoplasmic origin of these enzymes is established by comparing their activity with that of the mitochondrial β oxidation enzymes.
- Fiecchi,Galli-Kienle,Scala,Galli,Paoletti
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p. 516 - 528
(2007/10/09)
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