Synthesis of a new photo-cross-linking nucleoside analogue containing an aryl(trifluoromethyl)diazirine group: Application for EcoRII and MvaI restriction-modification enzymes
A new photo-cross-linking dU analog, 5-[4-(3-(trifluoromethyl)-3H- diazirin-3-yl)phenyl]-2'-deoxyuridine, was synthesized and incorporated into the recognition site of EcoRII and MvaI restriction-modification enzymes. The resulting base-modified 14-mer su