- Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED
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Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530–640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50–75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure–property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes.
- Butkevich, Alexey N.,Belov, Vladimir N.,Kolmakov, Kirill,Sokolov, Viktor V.,Shojaei, Heydar,Sidenstein, Sven C.,Kamin, Dirk,Matthias, Jessica,Vlijm, Rifka,Engelhardt, Johann,Hell, Stefan W.
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p. 12114 - 12119
(2017/09/14)
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