Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: A potent tool for the inhibition/isolation of serine esterases and proteases
6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of achymotrypsin.
Higson, Adrian P.,Ferguson, Michael A.J.,Nikolaev, Andrei V.