- Purification and characterization of the first archaeal glutamate decarboxylase from Pyrococcus horikoshii
-
Glutamate decarboxylase (GAD) from the archaeon Pyrococcus horikoshii was successfully expressed and purified, with the aim of developing a hyperthermostable GAD for industrial applications. Its biochemical properties were different from those reported for other GADs. The enzyme had broad substrate specificity, and its optimum pH and temperature were pH 8.0 and >97°C.
- Kim, Han-Woo,Kashima, Yasuhiro,Ishikawa, Kazuhiko,Yamano, Naoko
-
body text
p. 224 - 227
(2009/06/18)
-
- The application of glutamic acid α-decarboxylase for the valorization of glutamic acid
-
Glutamic acid is an important constituent of waste streams from biofuels production. It is an interesting starting material for the synthesis of nitrogen containing bulk chemicals, thereby decreasing the dependency on fossil fuels. On the pathway from glutamic acid to a range of molecules, the decarboxylation of glutamic acid to γ-aminobutyric acid (GABA) is an important reaction. This reaction, catalyzed by the enzyme glutamic acid α-decarboxylase (GAD) was studied on a gram scale. In this study, GAD was immobilized on Eupergit and in calcium alginate and its operational stability was determined in a buffer free system, using various reactor configurations. Immobilization was shown to increase the GAD stability. The conditions for the highest GABA production per gram of enzyme were determined by extrapolation of enzyme stability data. At 30 °C in a fed batch process this results in an average volumetric productivity of 35 kg m-3 hr-1. The cost of using GAD immobilized in calcium alginate was estimated as €5 per metric ton of product. Furthermore it was shown that the cofactor pyridoxal-5′-phosphate (PLP) could be regenerated by the addition of a small amount of α-ketoglutaric acid to the reactor. In conclusion the application of immobilized GAD in a fed batch reactor was shown to be a scalable process for the industrial production of GABA from glutamic acid. The Royal Society of Chemistry 2009.
- Lammens, Tijs M.,De Biase, Daniela,Franssen, Maurice C. R.,Scott, Elinor L.,Sanders, Johan P. M.
-
body text
p. 1562 - 1567
(2010/06/17)
-
- Synthese, activite antiparasitaire et antifongique de thioureines a motif aminoacide
-
Sixteen compounds, close to aryl or aralkylthioureines, with a β-alanine or γ-aminobutyric acid chain, were synthesized for in vitro screening towards 3 types of nematodes, 8 classes of opportunist germs and 34 classes of yeasts.Their anthelmintic activit
- Caujolle, R,Payard, M,Loiseau, PR,Amarouch, H,Linas, MD,et al.
-
p. 723 - 727
(2007/10/02)
-