Biosynthesis of glucose-d-13C6 from Hansenula polymorpha and methanol-13C
A simple and effective method for synthesis of glucose-d- 13C6 by fermentation using the methylotrophic yeast Hansenula polymorpha with 99% abundance methanol-13C is described. Using methanol-13C as a sole source of carbon, H. polymorpha can accumulate large amounts of α,α-trehalose-13C 12 under unfavourable growth conditions; the trehalose can then be hydrolysed to give glucose-d-13C6 with 98.5% abundance 13C.