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27580-13-8

NAPHTHOL AS-D PHOSPHATE is a fluorescent stain extensively utilized in biological and medical applications, particularly for detecting and quantifying alkaline phosphatase activity in enzyme-linked immunosorbent assays (ELISAs). It is renowned for its high sensitivity and stability, making it an indispensable reagent for identifying specific enzymes and markers in biological samples.

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  • 27580-13-8 Structure

  • Basic information

    1. Product Name: NAPHTHOL AS-D PHOSPHATE
    2. Synonyms: 2 NAPHTHO-O-TOLUIDINE-3-PHOSPHORIC ACID;NAPHTHOL AS-D PHOSPHATE;3-(phosphonooxy)-N-(o-tolyl)naphthalene-2-carboxamide;N-(2-Methylphenyl)-3-(phosphonooxy)-2-naphthalenecarboxamide
    3. CAS NO:27580-13-8
    4. Molecular Formula: C18H16NO5P
    5. Molecular Weight: 357.3
    6. EINECS: 248-542-9
    7. Product Categories: N/A
    8. Mol File: 27580-13-8.mol

  • Chemical Properties

    1. Melting Point: N/A
    2. Boiling Point: °Cat760mmHg
    3. Flash Point: °C
    4. Appearance: Peach powder
    5. Density: 1.456 g/cm3
    6. Refractive Index: 1.706
    7. Storage Temp.: N/A
    8. Solubility: N/A
    9. PKA: 0.80±0.30(Predicted)
    10. CAS DataBase Reference: NAPHTHOL AS-D PHOSPHATE(CAS DataBase Reference)
    11. NIST Chemistry Reference: NAPHTHOL AS-D PHOSPHATE(27580-13-8)
    12. EPA Substance Registry System: NAPHTHOL AS-D PHOSPHATE(27580-13-8)

  • Safety Data

    1. Hazard Codes: N/A
    2. Statements: N/A
    3. Safety Statements: N/A
    4. WGK Germany:
    5. RTECS:
    6. HazardClass: N/A
    7. PackingGroup: N/A
    8. Hazardous Substances Data: 27580-13-8(Hazardous Substances Data)

27580-13-8 Usage

Uses

Used in Research and Diagnostic Applications:
NAPHTHOL AS-D PHOSPHATE is used as a fluorescent stain for detecting and quantifying alkaline phosphatase activity in enzyme-linked immunosorbent assays (ELISAs). It is employed for its high sensitivity and stability, allowing for the accurate identification of specific enzymes and markers in biological samples.
Used in Combination with Other Substrates:
NAPHTHOL AS-D PHOSPHATE is used in conjunction with other substrates to achieve specific detection of alkaline phosphatase enzyme. This combination enhances the accuracy and reliability of the detection process, making it a valuable tool for research and diagnostic purposes.
Used in Biological and Medical Applications:
In the biological and medical fields, NAPHTHOL AS-D PHOSPHATE is used as a fluorescent stain for various applications, including the detection of certain enzymes and markers in biological samples. Its high sensitivity and stability make it an essential reagent for these applications.

Check Digit Verification of cas no

The CAS Registry Mumber 27580-13-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,5,8 and 0 respectively; the second part has 2 digits, 1 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 27580-13:
(7*2)+(6*7)+(5*5)+(4*8)+(3*0)+(2*1)+(1*3)=118
118 % 10 = 8
So 27580-13-8 is a valid CAS Registry Number.
InChI:InChI=1/C18H16NO5P/c1-12-6-2-5-9-16(12)19-18(20)15-10-13-7-3-4-8-14(13)11-17(15)24-25(21,22)23/h2-11H,1H3,(H,19,20)(H2,21,22,23)

27580-13-8SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 20, 2017

Revision Date: Aug 20, 2017

1.Identification

1.1 GHS Product identifier

Product name [3-[(2-methylphenyl)carbamoyl]naphthalen-2-yl] dihydrogen phosphate

1.2 Other means of identification

Product number -
Other names 3-[(2-methylphenyl)carbamoyl]-2-naphthyl dihydrogen phosphate

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:27580-13-8 SDS

27580-13-8Downstream Products

27580-13-8Relevant articles and documents

Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids

Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi

, p. 1347 - 1353 (2007/10/02)

A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.

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