- LIPIDOSOME PREPARATION, PREPARATION METHOD AND APPLICATION THEREOF
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The present invention discloses a liposome formulation, its preparation method, and its application in the treatment of diseases caused by abnormal gene expression. The liposome formulation comprises complementary cationic lipid pairs, phospholipids, and long-circulating lipids. The method of preparing the liposome formulation comprises: mixing the complementary cationic lipid pairs with the phospholipid and the long-circulating lipid to generate pre-formed vesicles; and then mixing the pre-formed vesicles with the nucleic acid solution to generate the liposome-nucleic acid formulation. This liposome formulation provided by the present invention is easily prepared; and in the treatment of diseases caused by abnormal gene expression, the liposome formulation can be used to deliver in vivo therapeutic agents, including nucleic acids.
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Paragraph 0045
(2015/12/20)
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- Synthesis and characteristics of biodegradable pyridinium amphiphiles used for in vitro DNA delivery
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Pyridinium amphiphiles have found practical application for the delivery of DNA into eukaryotic cells. A general synthetic method starting from (iso)nicotinoyl chloride has been devised for the preparation of pyridinium amphiphiles based on (bio)degradable esters, allowing structural variation both in the hydrophobic part and in the headgroup area. By means of differential scanning calorimetry, transmission electron microscopy and UV measurements, some characteristics, including hydrolytic behaviour, have been determined. In vitro transfection ability and toxicity have been determined using the eukaryotic COS-7 cell line. Wiley-VCH Verlag GmbH, 69451 Weinheim, Germany, 2002.
- Roosjen, Astrid,Smisterova, Jarmila,Driessen, Cecile,Anders, Joachim T.,Wagenaar, Anno,Hoekstra, Dick,Hulst, Ron,Engberts, Jan B. F. N.
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p. 1271 - 1277
(2007/10/03)
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