- Studies on the reaction between peptides or proteins with N-phosphoryl amino acids in aqueous solution
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In this article, it was found that N-phosphoryl α-amino acid could react with peptide or protein in aqueous solution to form the peptides or proteins increasing one acid amino residue. Through tandem-MS (MS/MS) and liquid chromatography-tandem mass spectrometry (LC/MS/MS), it was found that the added amino acid residue was located at the N-terminal of material peptides or proteins. Furthermore, the added amino acid residue was from the N-phosphoryl α-amino acid. In this reaction process, a possible mechanism was proposed that five-membered ring penta-coordinate phosphorous intermediate played the vital role in the formation of peptide bond. Copyright Taylor & Francis Group, LLC.
- Wang, Jia-Ning,Liu, Yan,He, Da-Cheng,Zhao, Yu-Fen
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experimental part
p. 764 - 772
(2009/04/06)
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