29701-07-3 Usage
Uses
Used in Pharmaceutical Industry:
KANAMYCIN B SULFATE is used as an antibiotic agent for treating various bacterial infections. Its potent antibacterial properties make it effective against a wide range of gram-negative and gram-positive bacteria, including those that are resistant to other antibiotics. This application is particularly important in the treatment of severe or life-threatening infections where other antibiotics may be ineffective.
Used in Research and Development:
In the field of research and development, KANAMYCIN B SULFATE is utilized as a tool to study the mechanisms of bacterial resistance and the development of new antibiotics. By understanding how this antibiotic interacts with bacterial cells, researchers can gain insights into the design of novel compounds with improved efficacy and reduced resistance potential.
Used in Veterinary Medicine:
KANAMYCIN B SULFATE is also employed in veterinary medicine as an antibiotic to treat bacterial infections in animals. Its broad-spectrum activity makes it suitable for treating a variety of bacterial infections in different species, including livestock, pets, and wildlife.
Used in Agricultural Applications:
In agriculture, KANAMYCIN B SULFATE is used to prevent and treat bacterial infections in crops and livestock. Its application helps to maintain the health of the plants and animals, leading to increased productivity and reduced economic losses due to disease.
Originator
Kanendomycin,Meiji Seika,Japan,1969
Manufacturing Process
200 liters of the medium containing 2.0% starch, 1.0% soybean meal, 0.05%
KCl, 0.05% MgSO4·7H2O, 0.3% NaCl, 0.2% NaNO3 was placed in the 400 liter
fermenter, the pH was adjusted to 7.5, and the medium was then sterilized
(pH after the sterilization was 7.0) for 30 minutes at 120°C, inoculated with
1,000 ml of 40 hour shake-cultured broth of S. kanamyceticus (a selected
subculture of K2-J strain) and tank-cultured at 27°-29°C. As antifoam,soybean oil (0.04%)and silicone (0.04%) were added. The broth after 48
hours was found to contain 250 mcg/ml of kanamycin.
A portion (950 ml) of the rich eluate was adjusted to pH 6.0 by the addition of
sulfuric acid. Ultrawet K (7.0 g) in 70 ml water was added slowly to the
neutralized eluate to precipitate kanamycin B dodecylbenzenesulfonate which
was collected by filtration after adding filter aid (Dicalite). The cake was
washed with water and extracted with 100 ml methanol. After filtering and
washing with methanol, sulfuric acid was added to the filtrate until no more
kanamycin B sulfate precipitated. After addition of an equal volume of acetone
to provide more complete precipitation, the kanamycin B sulfate was collected
by filtration, washed with methanol and dried in vacuo at 50°C.
Therapeutic Function
D-Streptamine, O-3-amino-3-deoxy-α-D-glucopyranosyl-(1-
6)-O-[2,6-diamino-2,6-dideoxy-α-D-glucopyranosyl-(1-4)]-2-deoxy
sulfate (1:1)
Purification Methods
A small quantity of kanamycin B (24mg) can be purified on a small Dowex-1 x 2 column (6 x 50mm); the required fraction is evaporated to dryness and the residue crystallised from EtOH containing a small amount of H2O. [Umezawa et al. Bull Chem Soc Jpn 42 537 1969.] It has been crystallised from H2O by dissolving ~1g in H2O (3mL), adding Me2NCHO (3mL) and setting aside at 4o overnight. The needles are collected and dried to constant weight at 130o. It has also been recrystallised from aqueous EtOH. It is slightly soluble in CHCl3 and isoPrOH. [IR: Wakazawa et al. J Antibiot 14A 180, 187 1961, Ito et al. J Antibiot 17 A 189 1964, Beilstein 18 III/IV 7631.]
Check Digit Verification of cas no
The CAS Registry Mumber 29701-07-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,9,7,0 and 1 respectively; the second part has 2 digits, 0 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 29701-07:
(7*2)+(6*9)+(5*7)+(4*0)+(3*1)+(2*0)+(1*7)=113
113 % 10 = 3
So 29701-07-3 is a valid CAS Registry Number.
InChI:InChI=1/C18H37N5O10.H2O4S/c19-2-6-11(26)12(27)9(23)17(30-6)32-15-4(20)1-5(21)16(14(15)29)33-18-13(28)8(22)10(25)7(3-24)31-18;1-5(2,3)4/h4-18,24-29H,1-3,19-23H2;(H2,1,2,3,4)/t4-,5+,6+,7+,8-,9+,10+,11+,12+,13+,14-,15+,16-,17+,18+;/m0./s1
