- The catalysed NADH reduction of resazurin to resorufin
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Details are reported on the mechanism whereby NADH can be used for the reduction of resazurin 1 to give the fluorescent product resorufin 2, a process requiring the use of a catalyst, such as N-methylphenazinium methosulfate 3.
- Candeias, Luis P.,MacFarlane, Donald P. S.,McWhinnie, Sean L. W.,Maidwell, Nicola L.,Roeschlaub, Carl A.,Sammes, Peter G.,Whittlesey, Rachel
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- A Simple and Efficient Flow Preparation of Pyocyanin a Virulence Factor of Pseudomonas aeruginosa
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The synthesis of the naturally occurring toxin pyocyanin has been realized in a short 4 step sequence. The key photochemical reaction and isolation of the final product have been facilitated by the use of flow chemistry techniques and immobilised reagents. Using these procedures gram quantities of pyocyanin were easily prepared in high yield and purity.
- Mortzfeld, Frederik B.,Pietruszka, J?rg,Baxendale, Ian R.
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supporting information
p. 5424 - 5433
(2019/06/13)
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- Hydrobromic acid-dimethyl sulfoxide reagent for dealkylation of 5,10-dialkyl-5,10-dihydrophenazines: Synthesis of 10-alkyl- 2(10H)-phenazinones
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By the reaction of 5,10-dialkyl-substituted 5,10- dihydrophenazine with hydrobromic acid in dimethyl sulfoxide at 90-110°, 10-alkyl-2(10H)-phenazinone was obtained as a major product. Brominated dihydrophenazine was isolated in the case of 1,6-dichloro-5,10-dimethyl-5,10-dihydrophenazine.
- Sugimoto, Akira,Yoshino, Yasuyuki,Watanabe, Ryo,Mizuno, Kazuhiko,Uehara, Kaku
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p. 1057 - 1064
(2007/10/03)
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- Synthesis of 5'-polyarene-tethered oligo-DNAs and the thermal stability and spectroscopic properties of their duplexes and triplexes
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Eleven different planar hydroxy alkylated polyarenes 1-11 with different geometry, bulk and electronic characteristics were synthesised, and used for tethering to the 5'-phosphate of a 9-mer and a 18-mer DNA sequences through solid-phase synthesis. The 5'-polyarene-tethered 9-mers 30-40 were tested for their ability to form stable duplexes with four complementary target DNA-strands 25-28 of different length. The 5'-polyarene-tethered 18-mers 44-54 were tested for their ability to form stable triplexes with a 24-mer duplex target 41+42. The T(m) measurements of duplexes at low salt and pH 7.3 showed that the angular nitro phenanthrene and phenanthrene conjugates 31 and 30 gave the highest duplex stabilisations with targets 25 (ΔT(m) 13.8°C and 11.8°C) and 26 (ΔT(m), 12.3°C and 11.9°C). With the mismatch sequence 28, only 30 and 31 gave a high ΔT(m), of 11.6°C and 10.8°C respectively, while lower ΔT(m), values were observed for other conjugates (ΔT(m), -4.0-5.0°C). The T(m) measurements of triplexes between 43-54 and duplex target 41+42 at low salt and pH 7.3, 6.5 and 6.0 without Mg2+ showed that the nitro phenanthrene conjugate 45 gave the best triplex stabilisation (ΔT(m), 4.1-5.4°C). The stabilisation of nitro phenanthrene conjugate 45 compared to phenanthrene conjugate 44 increased more remarkably when Mg2+ was present: 45 (ΔT(m), 15°C), 44 (ΔT(m), 10°C). These results imply that the electron density of the chromophore influences the π-π stacking interactions between the chromophore and nucleobases, and thereby influencing the duplex and triplex stability. Fluorescence measurements on single strand to double strand transition indicated that the 5'-tethered polyarenes are stacked only on the neighbouring nucleobases of the opposite strand. In case of 5'-9-N-ethylphenazinium conjugate 36, a comparative NMR and fluorescence measurement has unambiguously shown that the tethered phenazinium ion is indeed intercalated between the nucleotides of the opposite target strand 26. Thermodynamic calculations showed the most stable ΔG°(298K) for 30, 31(+targets 25, 26, 28) and 35, 36(+targets 25, 26) compared to the blank 29 ΔΔG°(298K) ~-10kJ mol-1). The non-palindromic target 27 was shown by T(m) measurements to form a stable tertiary structure, which was very little affected by addition of any 5'-tethered conjugate, thereby showing the importance of the tertiary structures of an in vivo antisense target and its implication in regard to its bioavailablity to complementary antisense probes.
- Puri, Nitin,Zamaratski, Edouard,Sund, Christian,Chattopadhyaya, Jyoti
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p. 10409 - 10432
(2007/10/03)
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