N-terminal derivatization of peptides with isothiocyanate analogues promoting edman-type cleavage and enhancing sensitivity in electrospray ionization tandem mass spectrometry analysis
N-terminal derivatization of peptides with Edman's reagent, phenyl isothiocyanate (PITC), promotes gas-phase Edman cleavage that yields abundant complementary b 1 and y n-1 ion pairs by tandem mass spectrometry (MS/MS). The formation