The invention concerns a novel chromogenic substrate based on Alizarin. The invention also concerns the uses to which this substrate can be put, and a formulation containing such a substrate. It consists therefore of a substrate to detect the presence of an enzyme activity, with the following general formula: in which: the two ketone groups of the central ring can be replaced by one group R11 (in the upper position), and by OH (in the lower position). R1 is a target part or H, and R2 is a target part or H, with at least one out of R1 and R2 being a target part, R3 is H, SO3H, Cl, Br, F, I, NO2, NH2, NR9R10, or an Acylamino Aminoaryl or Aminoacylamino group of the type NHCOX, with X being an Alkyl, Aryl or Aralkyl group or an a-amino acid residue such as Alanine, R4 is H, SO3H, Cl, Br, F, I, NO2, NH2, NR9R10, OH or an Acylamino Aminoaryl or Aminoacylamino group of the type NHCOX, with X being an Alkyl, Aryl or Aralkyl group or an α-amino acid such as Alanine, according to a modification, R3 and R4 form bonds with one another to create a ring with at least five sides, and preferably six sides. R5, R6, R7 and R8 each consist of one of the following atoms or groups of atoms: H, a halogen (particularly Cl or Br), OH, SO3H, or an Alkyl or Alkoxy group, and R9 a R10 are independently a Methyl, Alkyl, Aryl, Aralkyl group, or one (either R9 or R10) is a ring structure (Piperidine, Pyrrolidine, Morpholine, etc.) with the other (either R10 or R9) being a Hydrogen atom. The invention is particularly applicable in the field of diagnosis.