38483-26-0 Usage
Uses
Used in Biomedical Research:
Hydroethidine is used as a fluorescent probe for monitoring the production of reactive oxygen species in biological systems. Its application is crucial for understanding oxidative stress and its implications in various diseases and cellular processes.
Used in Flow Cytometry:
Hydroethidine is used as a flow cytometry indicator to measure the level of superoxide anion in cells. This technique allows for the rapid and accurate assessment of oxidative stress in cell populations, which is vital for research in cell biology and medicine.
Used in Cellular Imaging:
Hydroethidine is used as a cellular imaging agent to visualize the presence of superoxide anions within cells. The shift in fluorescence from blue to red provides a visual representation of ROS production, aiding researchers in studying the cellular response to various stimuli.
Used in Mitochondrial Function Studies:
Hydroethidine is used to investigate mitochondrial function and oxidative phosphorylation, as it undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. This application is essential for understanding the role of mitochondria in cellular metabolism and energy production.
Biochem/physiol Actions
Cell permeable: yes
Check Digit Verification of cas no
The CAS Registry Mumber 38483-26-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 3,8,4,8 and 3 respectively; the second part has 2 digits, 2 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 38483-26:
(7*3)+(6*8)+(5*4)+(4*8)+(3*3)+(2*2)+(1*6)=140
140 % 10 = 0
So 38483-26-0 is a valid CAS Registry Number.
InChI:InChI=1/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3/t21-/m1/s1
38483-26-0Relevant articles and documents
Monitoring the Instant Creation of a New Fluorescent Signal for Evaluation of DNA Conformation Based on Intercalation Complex
Uzumcu, Ahmet T.,Guney, Orhan,Okay, Oguz
, p. 1325 - 1332 (2018/09/25)
Here we report the monitoring the instant creation of a new fluorescent signal (FS) aroused from a positively charged water-soluble fluorogenic probe, ethidium bromide (EtBr) in the presence of a radical initiator, ammonium persulfate (APS) and an acceler
SYNTHESIS OF ANIONIC AND HYDROPHOBIC DERIVATIVES OF ETHIDIUM BROMIDE AS POTENTIAL NEW TRYPANOCYDES
Loccufier, J.,Schacht, E.
, p. 145 - 152 (2007/10/02)
This paper describes the synthesis of anionic and hydrophobic derivatives of 3,8-diamino-5-ethyl-6-phenyl phenantridium bromide (ethidium bromide, homidium).Evidence for regioselective acylation is provided.
