- Photoregulation of Gene Expression with Amantadine-Modified Caged siRNAs through Host–Guest Interactions
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RNA interference is an essential and powerful tool for targeting and verifying specific gene functions. Conditional control of small interfering RNA (siRNA) activity, especially using light activation, is a potential method for regulating target gene expr
- Zhang, Jinhao,Jing, Nannan,Fan, Xinli,Tang, Xinjing
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p. 14002 - 14010
(2020/10/02)
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- A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression
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Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3′-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, i
- Chen, Lei,Li, Jinbo,Sun, Yu.,Zhang, Yan
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p. 627 - 630
(2020/01/29)
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- Photochemical regulation of gene expression using caged siRNAs with single terminal Vitamin E modification
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Caged siRNAs with a single photolabile linker and/or vitamin E (vitE) modification at the 5′ terminal were rationally designed and synthesized. These virtually inactive caged siRNAs were successfully used to photoregulate both firefly luciferase and GFP g
- Ji, Yuzhuo,Yang, Jiali,Wu, Li,Yu, Lijia,Tang, Xinjing
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p. 2152 - 2156
(2016/02/18)
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- Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation
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Prenylation is an essential post-translational modification in all eukaryotes. Here we describe the synthesis of a 340-member library of peptides containing free C-termini on cellulose membranes. The resulting library was then used to probe the specificit
- Wang, Yen-Chih,Distefano, Mark D.
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supporting information; experimental part
p. 8228 - 8230
(2012/09/22)
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- Enantioconvergent hydrolysis of styrene epoxides by newly discovered epoxide hydrolases in mung bean
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Two novel epoxide hydrolases were discovered in mung bean (Phaseolus radiatus L.) for the first time, either of which can catalyze enantioconvergent hydrolysis of styrene epoxides. Their regioselectivity coefficients are more than 90% for the p-nitrostyrene oxide. Furthermore, the crude mung bean powder was also shown to be a cheap and practical biocatalyst, allowing a one-step asymmetric synthesis of chiral (R)-diols from racemic epoxides, in up to >99% ee and 68.7% overall yield (after recrystallization).
- Xu, Wei,Xu, Jian-He,Pan, Jiang,Gu, Qing,Wu, Xin-Yan
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p. 1737 - 1740
(2007/10/03)
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- Antibacterial Benzisoxazolones. An Unusual Rearrangement Product from o-Nitrostyrene Oxide en Route to the Photolabile Carbonyl Protecting Group (o-Nitrophenyl)ethylene Glycol
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In the process of synthesizing the known, photolabile protecting group (o-nitrophenyl)ethylene glycol, we uncovered an acid-mediated rearrangement of o-nitrostyrene oxide to 1-(hydroxymethyl)-2,1-benzisoxazol-3(1H)-one.The structure was ascertained by IR, UV, 1H NMR, 13C NMR, MS, and X-ray crystallography.Also the benzisoxazolone was prepared by independent synthesis from an o-nitrobenzoate.Another novel transformation uncovered was the conversion of o-nitrostyrene oxide to 2-(o-nitrophenyl)ethanol with use of potassium hydroxide and 18-crown-6.Similiar chemistry was insignificant with p-nitrostyrene oxide.The benzisoxazolone exhibited antibacterial and antileukemic activity (in vitro).
- Wierenga, Wendell,Harrison, Allen W.,Evans, Bruce R.,Chidester, Connie G.
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p. 438 - 442
(2007/10/02)
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- o-Nitrophenylethylene glycol as photoremovable protective group for aldehydes and ketones: syntheses, scope, and limitations
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The preparation of o-nitophenylethylene glycol is described along with its application as a photolabile protective group for aldehydes and ketones.Formation of the acetals and ketals is achieved in good to high yields in the usual manner and deprotection is carried out in fair to high yield, by photolysis at 350 nm in an inert solvent such as benzene.Because of the particular nature of the present protective group, its stability to basic and acidic conditions has been examined and is reported to complete the scope and limitations aspect.From a mechanistic point of view, the isolation and characterisation of o-nitroso-α-hydroxyacetophenone as the spent reagent demonstrates a mechanistic link with the known o-nitrobenzaeldehyde to o-nitrobenzoic acid photorearrangement.
- Gravel, D.,Herbert, J.,Thoraval, D.
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p. 400 - 410
(2007/10/02)
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- EPOXIDATION D'ALDEHYDES ET DE CETONES PAR UN PROCEDE DE TRANSFERT SOLIDE-LIQUIDE
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The condensation reaction of trimethylsulfonium iodide with variously functionalized aldehydes and ketones in the presence of solid potassium hydroxide in acetonitrile leads to the selective formation of the corresponding epoxides in high yields.The method used calls upon a solid-liquid transfer process.
- Borredon, Elisabeth,Delmas, Michel,Gaset, Antoine
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p. 5283 - 5286
(2007/10/02)
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- NEW METHODS AND REAGENTS IN ORGANIC SYNTHESIS. 11. REACTION OF TRIMETHYLSILYLDIAZOMETHANE WITH AROMATIC ALDEHYDES
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Trimethylsilyldiazomethane adds to aromatic aldehydes in the presence of triethylamine in methanolic solution to give epoxides and homologous compounds.
- Hashimoto, Norio,Aoyama, Toyohiko,Shioiri, Takayuki
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p. 975 - 979
(2007/10/02)
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