- Immunophotoaffinity labeling of binders of 1-methyladenine, the oocyte maturation-inducing hormone of starfish
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Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary and resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative 1-MeAde receptors on the oocyte surface have been suggested, but not yet been biochemically characterized. Immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection, was attempted to detect unknown 1-MeAde binders including putative maturation-inducing hormone receptors in starfish oocytes. When the oocyte crude membrane fraction or its Triton X-100/EDTA extract was incubated with N6-[6-(5-azido-2-nitrobenzoyl)aminohexyl]carboxamidomethyl-1-methyladenine and then photo-irradiated, followed by western blotting with antibody that was raised against a 1-MeAde hapten, a single band with Mr of 47.5?K was detected. The band was lost when extract was heated at 100?°C. A similar 47.5?K band was detected in the crude membrane fraction of testis as well. Upon labeling with whole cells, this band was detected in immature and maturing oocytes, but only faintly in mature oocytes. As judged from these results, this 1-MeAde binder might be a possible candidate of the starfish maturation-inducing hormone receptors.
- Toraya, Tetsuo,Kida, Tetsuo,Kuyama, Atsushi,Matsuda, Shinjiro,Tanaka, Seiichi,Komatsu, Yo,Tsurukai, Taro
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- Nicotinic acid adenine dinucleotide phosphate analogues substituted on the nicotinic acid and adenine ribosides. Effects on receptormediated Ca2+ release
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Nicotinic acid adenine dinucleotide phosphate (NAADP) is a Ca2+ releasing intracellular second messenger in both mammals and echinoderms. We report that large functionalized substituents introduced at the nicotinic acid 5-position are recognize
- Trabbic, Christopher J.,Zhang, Fan,Walseth, Timothy F.,Slama, James T.
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p. 3593 - 3610
(2015/05/05)
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- Convenient synthesis of 8-amino-2′-deoxyadenosine
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We studied the behaviour of 8-azido-2′-deoxyadenosine and 8-bromo-2′-deoxyadenosine in aqueous solutions of ammonia and primary and secondary amines. Unexpectedly, 8-Azido-2′-deoxyadenosine is converted to 8-amino-2′-deoxyadenosine in excellent yields. The use of this reaction for the preparation of 8-aminoadenine derivatives needed for the preparation of oligonucleotides carrying 8-aminoadenine is discussed.
- Frieden, Miriam,Avino, Anna,Eritja, Ramon
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p. 193 - 202
(2007/10/03)
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- Synthesis of some nucleoside analogues via azido intermediates
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Synthesis of some nucleosides via the intermediates 4-azido- ·and 6-azido-pyrimidines, 8-azido-purines and 2-methylthio-pyrimidines has been described.
- Sayed Ahmed
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p. 595 - 597
(2007/10/03)
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