- Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk
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A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose - hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.
- Nishimoto, Mamoru,Kitaoka, Motomitsu
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p. 2101 - 2104
(2008/02/13)
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