- Yohimbine hapten YHA, artificial antigen and antibody thereof, and preparation and application thereof
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The invention discloses a yohimbine hapten YHA, an artificial antigen and an antibody thereof as well as preparation and application thereof. The preparation method comprises preparing a yohimbine hapten YHA, wherein the structural formula of the yohimbine hapten YHA is shown as a formula (I); preparing a yohimbine artificial antigen and a yohimbine antibody YHA-IgG by using the hapten. The antibody YHA-IgG has high detection sensitivity and strong specificity for yohimbine, the half inhibition concentration is 0.50 mu g/kg, the detection range for yohimbine is 0.12-2.18 mu g/kg, the lowest detection limit is 1.13 mu g/kg, and the detection requirements are met; besides, the preparation methods of the yohimbine hapten YHA, the yohimbine artificial antigen and the yohimbine antibody YHA-IgGare simple and low in cost, and a core raw material is provided for establishing an immunological detection method of specific yohimbine; therefore, the yohimbine antibody YHA-IgG has a wide application prospect in detection of yohimbine.
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Paragraph 0041-0044
(2020/08/22)
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