- Nutritional traits of bean (Phaseolus vulgaris) seeds from plants chronically exposed to ozone pollution
-
The effect of chronic exposure to ozone pollution on nutritional traits of bean (Phaseolus vulgaris L. cv. Borlotto Nano Lingua di Fuoco) seeds from plants grown in filtered and nonfiltered open-top chambers (OTCs) has been investigated. Results showed th
- Iriti, Marcello,Di Maro, Antimo,Bernasconi, Silvana,Burlini, Nedda,Simonetti, Paolo,Picchi, Valentina,Panigada, Cinzia,Gerosa, Giacomo,Parente, Augusto,Faoro, Franco
-
supporting information; experimental part
p. 201 - 208
(2009/05/31)
-
- Separation of Δ5- and Δ7-phytosterols by adsorption chromatography and semipreparative reversed phase high-performance liquid chromatography for quantitative analysis of phytosterols in foods
-
A method for the separation, isolation, and identification of phytosterols was developed. A commercial phytosterols mixture, Generol 95S, was fractionated first by adsorption silica gel column chromatography and then separated by means of a semipreparative reverse phase high-performance liquid chromatography fitted with a Polaris C8-A column (250 mm x 10 mm i.d., 5 μm) using isocratic acetonitrile:2-propanol:water (2:1:1, v/v/v) as the mobile phase. Milligram scales of six individual phytosterols, including citrostadienol, campesterol, β-sitosterol, δ7-avenasterol, Δ7-campesterol, and Δ7-sitosterol, were obtained. Purities of these isolated sterols were 85-98%. Relative response factors (RRF) of these phytosterols were calculated against cholestanol as an authentic commercial standard. These RRF values were used to quantify by gas chromatography-mass spectrometry (GC-MS) the phytosterols content in a reference material, oils, and chocolates.
- Zhang, Xin,Cambrai, Amandine,Miesch, Michel,Roussi, Stamatiki,Raul, Francis,Aoude-Werner, Dalal,Marchioni, Eric
-
p. 1196 - 1202
(2007/10/03)
-
- Phytosterols in sea buckthorn (Hippophae rhamnoides L.) berries: Identification and effects of different origins and harvesting times
-
Sterols in seeds, pulp/peel fractions, and whole berries of sea buckthorn (Hippophae rhamnoides L.) samples belonging to two major subspecies (sinensis and rhamnoides) from Finland and China were analyzed as TMS derivatives by gas chromatography-mass spectrometry after saponification of the oils. The total sterol contents in the seeds, the fresh pulp/peel, and the whole berries were 1200-1800, 240-400, and 340-520 mg/kg, respectively. The corresponding values in the extracted oils were 12-23, 10-29, and 13-33 g/kg. Sitosterol constituted 57-76 and 61-83%, respectively, of the seed and pulp/peel sterols. The sterol content and composition showed little variation between subspecies and collection sites. Different harvesting dates showed significant effects on the levels of some sterols both in the seeds and in the pulp/peel. The sterol profiles obtained are useful for characterizing sea buckthorn and detecting adulterations of the valuable oils. The information provided by the present investigation is also important for further chemical investigation of sea buckthorn sterols and industrial utilization of the berries as a raw material of functional foods.
- Yang,Karlsson,Oksman,Kallio
-
p. 5620 - 5629
(2007/10/03)
-