Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification
Due to the lowered pKa of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.
Ring, Christine M.,Iqbal, Emil S.,Hacker, David E.,Hartman, Matthew C. T.,Cropp, T. Ashton
supporting information
p. 4536 - 4539
(2017/07/10)
Photochemical decomposition of diazonium fluoroborates. Application to the synthesis of ring-fluorinated imidazoles.