6035-45-6

Basic information

• Product Name: Calcium folinatc
• Synonyms: FOLINIC ACID CALCIUM CELL CULTURE TESTED;Folinic acid, calcium salt pentahydrate, 95.0-105.0%;CALCIUM FOLINATE(FOLINIC ACID)(RG);Calcium folinatc;Folinic acid calcium salt;Leucovorin calcium pentahydrate;NSC-3590;Folinic acid, calcium salt pentahydrate,95-105% %
• CAS NO: 6035-45-6
• Molecular Formula: C₂₀H₂₁N₇O₇*Ca
• Molecular Weight: 511.5
• Product Categories: Miscellaneous Biochemicals;Chiral Reagents;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Calcium, Calcium Folinatc, Citrovorum Factor;API
• Mol File: 6035-45-6.mol

Chemical Properties

• Melting Point: 240-250°C
• Appearance: Yellow-white to yellow/powder
• Storage Temp.: Store at RT.
• Solubility: insoluble in DMSO; insoluble in EtOH; ≥15.04 mg/mL in H2O with gentle warming
• CAS DataBase Reference: Calcium folinatc(CAS DataBase Reference)
• NIST Chemistry Reference: Calcium folinatc(6035-45-6)
• EPA Substance Registry System: Calcium folinatc(6035-45-6)

Safety Data

• Hazard Codes: Xn
• Statements: 36/37/38-42/43
• Safety Statements: 26-36
• WGK Germany: 3
• Hazardous Substances Data: 6035-45-6(Hazardous Substances Data)

Usage

Uses

Used in Pharmaceutical Industry:
Calcium folinate is used as an antianemic agent for the treatment of anemia caused by folic acid deficiency. It helps in the production of red blood cells and supports the body's ability to fight anemia.
Used in Oncology:
Calcium folinate is used as a chemosensitizer in the treatment of certain types of cancer, particularly in combination with other chemotherapeutic agents like 5-FU (5-fluorouracil). It enhances the efficacy of these drugs by increasing their sensitivity to the cancer cells, leading to improved treatment outcomes.
Used in Drug Metabolism:
Calcium folinate is used as a supplement in the treatment of megaloblastic anemia caused by inborn errors of folic acid metabolism. It aids in the proper functioning of the body's metabolic processes and supports overall health.
Used in Research and Development:
Calcium folinate is also used in research and development for studying the role of folic acid in various biological processes and its potential applications in the development of new drugs and therapies.

Biological Activity

human lymphoid cell lines were studied for leucovorin requirements to protect from methotrexate (mtx)-induced growth suppression. over a 72h continuous exposure leucovorin provided better protection to the cell lines laz-007 and raji than to the cell line

InChI:InChI=1/C20H23N7O7.Ca.5H2O/c21-20-25-16-15(18(32)26-20)27(9-28)12(8-23-16)7-22-11-3-1-10(2-4-11)17(31)24-13(19(33)34)5-6-14(29)30;;;;;;/h1-4,9,12-13,22H,5-8H2,(H,24,31)(H,29,30)(H,33,34)(H4,21,23,25,26,32);;5*1H2/q;+2;;;;;/p-2/t12?,13-;;;;;;/m0....../s1

Synthetic route

Leucovorin Calcium (6035-45-6) → folinic acid (58-05-9)

Conditions	Yield
With hydrogenchloride In water at 6 - 10℃; for 4h; pH=2.8 - 3.2;	n/a
With acetic acid In water at 2 - 12℃; pH=2.5 - 3.5;

Leucovorin Calcium (6035-45-6) → leucovorin zinc

Conditions	Yield
With zinc acetate dihydrate In water at 40℃;	2.5 g

Downstream Products

• 58-05-9 folinic acid 

Relevant articles and documents

Process for the preparation and separation of diastereomeric salts of folinic acid

A process for the preparation, separation and purification of (6S) and (6R) diastereomers of folinic acid salts with at least dibasic amines is disclosed, which process comprises the hydrolysis of (6RS)-5,10-methenyl-5,6,7,8-tetrahydrofolic acid chloride hydrochloride with an at least dibasic amine and the subsequent separation of the diasteromeric salts.

Process for separating folinic acid

Preparing (6S)-folinic acid and its salts, comprising recrystallization of alkaline-earth salts of (6R,S)-folinic acid and, where necessary, liberation of the acid from the alkaline-earth folinates and/or, where necessary, conversion into the alkali salts by at least one recrystallization in the presence of a base. Calcium-, magnesium-, potassium- and sodium-(6S)-folinates and (6S)-folinic acid are prepared.