- Elucidating the chemical and biochemical applications of Citrus sinensis-mediated silver nanocrystal
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Synthesis of nanoparticles using biodegradable source is safer and echo-friendly. Here, we describe the synthesis of polycrystalline silver nanocrystals using Citrus sinensis acting as both reducing and capping agents. After exposing the silver ions to orange extract, rapid reduction of silver ions led to the formation of stable silver nanocrystals due to the reducing and stabilizing properties of orange fruit juice. The synthesized silver nanocrystals were characterized using various analytical techniques like UV–vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscope (SEM) and transmission electron microscopy (TEM). The biochemical activity of the synthesized nanocrystals was studied in the light of affinity to bovine serum albumin using several biophysical methods like absorbance, fluorescence and circular dichroism spectroscopy. Cytotoxic activity of these nanocrystals was also studied against Hep-2 cell line using fluorescence microscopy. It was also found that the synthesized nanocrystals can sense mercuric ion down to 50 μM in the presence of a number of cations. Furthermore, we established that the silver nanoparticles can effectively catalyse the reduction of methylene blue by ascorbic acid. The present study will enrich our knowledge on the chemical and biochemical activities of green-synthesized silver nanocrystals.
- Siddiqui, Nurunnesa,Masum, Abdulla Al,Uddin, Md. Raihan,Mandal, Sukhendu,Sengupta, Mohana,Islam, Md. Maidul,Mukhopadhyay, Subrata
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p. 4863 - 4874
(2019/01/24)
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- Effects of ionic surfactants and cyclodextrins on hydride-transfer reaction of l-Benzyl-l,4-dihydronicotinamide with methylene blue
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The kinetics of the hydride-transfer reaction between methylene blue (MB+) and 1 -benzyl-1,4-dihydronictinamide (BNAH) were studied in media containing cyclodextrins (β- and γ-CD) and surfactants (sodium dodecyl sulfate (SDS), dodecyltrimethylammonium bromide, tetradecyltrimethylammonium bromide, and hexadecyltrimethylammonium bromide). Cationic surfactants decreased the apparent first-order rate constant (k obsd) above the cmc, while SDS increased kobsd just above the cmc and then decreased kobsd with increasing surfactant concentration. This behavior for cationic surfactants was typical of micellar effects due to a separation of the reactants by the micelles. BNAH associated with micelles, whereas MB+ ions were repelled from the cationic interface of the micelles. Binding of BNAH and MB to the same SDS micelle enhanced the reaction, but dilution of reagents within the micellar interface with the increase in [SDS] caused a decrease in Kobsd. In β-CD-cationic surfactant mixtures, the results were interpreted in terms of the model which takes into account the formation of CD-BNAH, CD-MB+, and CD-surfactant complexes and the association of BNAH with micelles. The decrease in Kobsd with increasing surfactant concentration observed in γ-CD-cationic surfactant mixtures can be explained by the decrease in the concentration of free γ-CD by the formation of 1:1 and 2:1 complexes of surfactant monomer with β-CD.
- Matsumoto, Takeshi,Liu, Yingjin,Sueishi, Yoshimi,Yamamoto, Shunzo
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experimental part
p. 1383 - 1390
(2009/06/19)
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- Kinetic study on the reversible hydride transfer between methylene blue and thionine
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The kinetics of the hydride transfer reaction between leuco methylene blue and thionine was investigated spectrophotometrically by means of the stopped-flow technique. Leuco methylene blue and leuco thionine were produced by photoreduction of methylene bl
- Liu,Yamamoto,Fujiyama,Sueishi
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p. 2367 - 2371
(2007/10/03)
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