Synthesis and Enzymatic Carboxylation of a Biotin-containing Peptide Representing the Coenzyme Binding Site of E. coli Acetyl-CoA Carboxylase
A biotin-containing pentapeptide Boc-Glu-Ala-Met-Bct-Met (1) that corresponds to the coenzyme binding site of E. coli acetyl-CoA carboxylase has been prepared.Peptide 1 as well as free biotin served as a substrate for the carboxylation reaction catalyzed