Rerouting and Improving Dauc-8-en-11-ol Synthase from Streptomyces venezuelae to a High Yielding Biocatalyst
The dauc-8-en-11-ol synthase from Streptomyces venezuelae was investigated for its catalytic activity towards alternative terpene precursors, specifically designed to enable new cyclisation pathways. Exchange of aromatic amino acid residues at the enzyme surface by site-directed mutagenesis led to a 4-fold increase of the yield in preparative scale incubations, which likely results from an increased enzyme stability instead of improved enzyme kinetics.
Lauterbach, Lukas,Hou, Anwei,Dickschat, Jeroen S.
supporting information
p. 7923 - 7929
(2021/04/21)
Alkenoyl-cyclohexadienes
New cycloaliphatic unsaturated ketones and their use as perfuming and odour-modifying agents in the manufacture of perfumes and perfumed products, and as flavouring and taste-modifying agents in the preparation of foodstuffs in general and imitation flavours for foodstuffs, beverages, animal feeds, pharmaceutical preparations and tobacco products. Methods for the preparation of said cycloaliphatic unsaturated ketones.
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(2008/06/13)
Novel synthesis of dienones and enones from propargyl alcohols and allyl alcohols with 2,2 dimethoxypropane: synthesis of ionone and irone