- Method for producing 3-oxoadipic acid
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A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus Serratia, microorganisms belonging to the genus Corynebacterium, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Shimwellia, microorganisms belonging to the genus Planomicrobium, microorganisms belonging to the genus Nocardioides, microorganisms belonging to the genus Yarrowia, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Rhodosporidium, microorganisms belonging to the genus Streptomyces, and microorganisms belonging to the genus Microbacterium.
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Page/Page column 11-12
(2020/12/18)
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- ALTERNATIVE PATHWAYS TO ADIPIC ACID BY COMBINED FERMENTATION AND CATALYTIC METHODS
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Processes process for producing adipate or adipic acid using biological pathways and chemical catalyzes are disclosed. Homocitric acid may be a substrate in reaction pathways leading to adipic acid or a salt thereof.
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Paragraph 0132
(2015/09/22)
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- In vitro reconstitution of the catabolic reactions catalyzed by PcaHG, PcaB, and PcaL: The protocatechuate branch of the β-ketoadipate pathway in Rhodococcus jostii RHA1
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The β-ketoadipate pathway is a major pathway involved in the catabolism of the aromatic compounds in microbes. The recent progress in genome sequencing has led to a rapid accumulation of genes from the β-ketoadipate pathway in the available genetic database, yet the functions of these genes remain uncharacterized. In this study, the protocatechuate branch of the β-ketoadipate pathway of Rhodococcus jostii was reconstituted in vitro. Analysis of the reaction products of PcaHG, PcaB, and PcaL was achieved by high-performance liquid chromatography. These reaction products, β-ketoadipate enol-lactone, 3-carboxy-cis,cis-muconate, γ-carboxymuconolactone, muconolactone, and β-ketoadipate, were further characterized using LC-MS and nuclear magnetic resonance. In addition, the in vitro reaction of PcaL, a bidomain protein consisting of γ-carboxy-muconolactone decarboxylase and β-ketoadipate enol-lactone hydrolase activities, was demonstrated for the first time. This work provides a basis for analyzing the catalytic properties of enzymes involved in the growing number of β-ketoadipate pathways deposited in the genetic database.
- Yamanashi, Tomoya,Kim, Seung-Young,Hara, Hirofumi,Funa, Nobutaka
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p. 830 - 835
(2015/10/05)
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- Rapid Biodegradation of Aniline by Frateuria species ANA-18 and Its Aniline Metabolism
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A bacterial strain, ANA-18, was isolated from soil, when aniline was provided as a sole source of carbon and nitrogen at pH 5.5.The isolate belongs to a Frateuria species.Frateuria sp.ANA-18 was able to grow on aniline at pH 4.0 to 7.0 and readily degrated it.This bacterium decomposed aniline more rapidly than Rhodococcus erythropolis AN-13 reported previously.Resting cells of aniline-grown Frateuria sp.ANA-18 had 9-fold the oxidizing activity for aniline of those of R. erythropolis AN-13.The metabolic pathway for mineralization of aniline by Frateuria sp.ANA-18 was the same as that proposed for R. erythropolis AN-13.
- Aoki, Kenji,Ohtsuka, Kotaro,Shinke, Ryu,Nishira, Hiroshi
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p. 865 - 872
(2007/10/02)
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