- Synthesis and characterization of an industrially significant ionic liquid and its inclusion complex with β-cyclodextrin and its soluble derivative for their advanced applications
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Thermochromic also solvatochromic ionic liquid surfactant was synthesized and characterised. The surface activity, size and stability were estimated by surface tension, SEM and DLS techniques. Inclusion complexes construction of the synthesized compound with the β-cyclodextrin and derivative was explored by various techniques. Conductance, surface tension trial & Jobs obtained from the UV–vis spectra proved the formation of 1:1 inclusion complexes between the surfactant and cyclodextrins. Binding constants and thermodynamic parameters of the inclusion of the guest DMPI with both hosts were determined with UV–vis and sophisticated fluorimetric analysis. Anti bacterial properties of DMPI and its both inclusion complexes were analyzed.
- Ghosh, Raja,Roy, Niloy,Saha, Subhadeep,Das, Samir,Kumar Barman, Biraj,Roy, Debadrita,Kumar Dakua, Vikas,Nath Roy, Mahendra
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- Novel alkyl chain-based fluorescent probes with large Stokes shifts used for imaging the cell membrane and mitochondria in different living cell lines
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Fluorescent dyes with large Stokes shifts play a key role in developing multi-purpose fluorescent probes for a wide variety of targets. In this study, we developed two novel alkyl chain-based fluorescent probes (CA-C12 and CA-C2) with large Stokes shifts. The alkyl chain length of the probes affect the membrane permeability, and hence both probes can be successfully applied for sensing the cell membrane and mitochondria in different living cell lines. Furthermore, the probes CA-C12 and CA-C2 exhibited large Stokes shifts and excellent photostability in the different cell lines. The fluorescent dyes with large Stokes shifts were expected to have broader applications for developing various fluorescent probes with excellent optical properties.
- Meng, Fangfang,Liu, Yong,Niu, Jie,Lin, Weiying
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- Interface targeting type mitochondrial probe as well as preparation method and application thereof
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The invention discloses an interface targeting type mitochondrial probe as well as a preparation method and application thereof in living cells and tissues, and belongs to the technical field of mitochondrial fluorescent probes. The structural formula of the interface targeting type mitochondrial probe is shown in the specification. The chemical name of the interface targeting type mitochondrial probe is (E)-4-(2-(7-diethylamino-coumarin) vinyl)-1-dodecyl pyridine iodate. The probe is named as CVP-12. The interface targeting type mitochondrial probe has the characteristics of no need of washing, high selectivity, good biocompatibility, excellent membrane permeability and low cytotoxicity, and has good application prospects in the field of biomarkers.
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Paragraph 0038; 0042
(2020/07/15)
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- A side-chain engineering strategy for constructing fluorescent dyes with direct and ultrafast self-delivery to living cells
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Organic fluorescent dyes with excellent self-delivery to living cells are always difficult to find due to the limitation of the plasma membrane having rigorous selectivity. Herein, in order to improve the permeability of dyes, we utilize a side-chain engineering strategy (SCES): adjusting the side-chain length of dyes to fine-tune the adsorption and desorption processes on the membrane-aqueous phase interfaces of the outer and inner leaflets of the plasma membrane. For this, a family of fluorescent derivatives (SPs) was prepared by functionalizing a styryl-pyridinium fluorophore with alkyl side-chains containing a different carbon number from 1 to 22. Systematic experimental investigations and simulated calculations demonstrate that the self-delivery rate of SPs with a suitable length side-chain is about 22-fold higher in SiHa cells and 76-fold higher in mesenchymal stem cells than that of unmodified SP-1, enabling cell-imaging at an ultralow loading concentration of 1 nM and deep penetration in turbid tissue and in vivo. Moreover, the SCES can even endow a membrane-impermeable fluorescent scaffold with good permeability. Further, quantitative research on the relationship between Clog?P and cell permeability shows that when Clog?P is in the range of 1.3-2.5, dyes possess optimal permeability. Therefore, this work not only systematically reports the effect of side-chain length on dye delivery for the first time, but also provides some ideal fluorescent probes. At the same time, it gives a suitable Clog?P range for efficient cellular delivery, which can serve as a guide for designing cell-permeant dyes. In a word, all the results reveal that the SCES is an effective strategy to dramatically improve dye permeability.
- Guo, Lifang,Li, Chuanya,Shang, Hai,Zhang, Ruoyao,Li, Xuechen,Lu, Qing,Cheng, Xiao,Liu, Zhiqiang,Sun, Jing Zhi,Yu, Xiaoqiang
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p. 661 - 670
(2020/01/31)
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- Non-reactive mitochondrial tracking fluorescent probe IVPI-12 and applications thereof
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The invention discloses a non-reactive mitochondrial tracking fluorescent probe IVPI-12, which has a C12 lalkyl chain in the chemical structure, and is an indole pyridine salt compound having a structure represented by a formula (I). The invention further discloses applications of the probe in labeling or displaying of the distribution of mitochondria in cells, and in mitochondrial autophagy observation. According to the present invention, the experiment results prove that the probe can stain mitochondria in normal living cells, and especially can be fixed on mitochondria when the mitochondrial membrane potential is reduced or disappears, such that the results show that the probe can track the dynamic changes of mitochondria; the probe further has good two-photon properties, and can be used for deep tissue imaging; and the probe has low toxicity, can real-timely track the dynamic process of mitochondrial autophagy, and has broad application prospect in the field of fluorescent biomarkers.
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Paragraph 0012; 0031
(2019/02/13)
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- Non-reactive mitochondrial tracking fluorescent probe containing C12 lalkyl chain, and applications thereof
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The invention discloses a non-reactive mitochondrial tracking fluorescent probe containing a C12 lalkyl chain, and applications of the probe in labeling or displaying of the distribution of mitochondria in cells, and in mitochondrial autophagy observation, wherein the fluorescent probe is a carbazole pyridine salt compound having a structure represented by a formula (I). According to the present invention, the probe can stain mitochondria in normal living cells, and especially can be fixed on mitochondria when the mitochondrial membrane potential is reduced or disappears, such that the resultsshow that the probe can track the dynamic changes of mitochondria; the probe further has good two-photon properties, and can be used for deep tissue imaging; and the probe has low toxicity, can real-timely track the dynamic process of mitochondrial autophagy, and has broad application prospect in the field of fluorescent biomarkers.
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Paragraph 0033-0036
(2019/02/13)
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- A photostable fluorescent probe for rapid monitoring and tracking of a trans-membrane process and mitochondrial fission and fusion dynamics
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Rapid monitoring and tracking of a trans membrane process and mitochondrial fission and fusion dynamics play critical roles in judging the occurrence and development of disease, and can give insights for studying apoptosis and cell degeneration. However, the existing probes are not capable of rapid monitoring and tracking the above dynamics process. To solve this problem, we develop a unique functional mitochondria probe containing long alkyl chains, 3,5-bis((E)-2-(pyridin-4-yl)vinyl)-1H-indole monoiodide (MT-PVIM), which is capable of rapid real-time imaging and tracking mitochondrial fission and fusion dynamics. In addition, compared with a commercially available mitochondrial probe MTR, the MT-PVIM probe has excellent specificity to mitochondria with outstanding tolerance of micro-environmental changes, thus representing a potential candidate as a tracking agent for apoptosis studies. The good performance of our proposed approach demonstrates that this strategy might open up new opportunities for the development of rapid image mitochondria-targetable molecular tools for bioanalytical and biomedical applications.
- Liu, Yong,Meng, Fangfang,Tang, Yonghe,Yu, Xiaoqiang,Lin, Weiying
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supporting information
p. 3726 - 3731
(2016/05/19)
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- Two-photon deep red emission fluorescent probe for imaging cell membranes in tissues based on molecular rotors
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The invention discloses a two-photon deep red emission fluorescent probe for imaging cell membranes in tissues based on molecular rotors. The chemical name of the two-photon deep red emission fluorescent probe is N,N-di((4-(2(minute)-(4(second)-dodecyl)py
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Paragraph 0030; 0031; 0032
(2016/11/09)
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- Long Chain Heterocyclic Dyes. Part One. Hydrophobic Structures
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A series of polymethine dyes and of their precursors, containing long and short alkyl chains, was prepared and their chemical and spectroscopic (uv, 1H-nmr) properties were briefly discussed.The electronic absorption spectra of the dyes in aqueous medium
- Barni, Ermanno,Savarino, Piero,Larovere, Raffaella,Viscardi, Guido,Pelizzeti, Ezio
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p. 209 - 222
(2007/10/02)
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