- Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques
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For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.
- Seliger, Hartmut,Bach, Trung-Chinh,Siewert, Gerhard,Boidol, Werner,Toepert, Michael,et al.
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p. 835 - 853
(2007/10/02)
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- Nucleosides and Nucleotides. Part 17. A Simple Preparation of Protected Deoxynucleoside-3'-phosphates
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A simple method for the preparation of fully protected 2'-deoxynucleoside-3'-phosphates is described.The main step, phosphorylation of partially protected deoxynucleosides, is performed with the monofunctional reagent p-chlorophenyl(2-cyanoethyl)phosphochloridate (2) in dioxane.This reagent is quickly prepared from readily accessible p-chlorophenyl phosphodichloridate by reaction with 3-hydroxypropionitrile and triethylamine in dioxane or ether.The crude reagent is used directly for phosphorylation.After chromatography, protected deoxynucleoside-3'-phosphonates were isolated in yields of 80-87percent .The method described here, which has been optimized in detail, represents a simple alternative to published methods.
- Bernardini, Silvio De,Waldmeier, Felix,Tamm, Christoph
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p. 2142 - 2147
(2007/10/02)
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- PREPARATION RAPIDE DES MONONUCLEOSIDES-3' MONOPHOSPHATE PROTEGES UTILISABLES EN SYNTHESE OLIGONUCLEOTIDIQUE
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Fully protected mononucleotides are prepared by the action of parachlorophenylphosphodichloridite in THF on the corresponding nucleosides followed by oxidation with iodine.
- Molko, D.,Derbyshire, R. B.,Guy, A.,Roget, A.,Teoule, R.
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p. 2159 - 2160
(2007/10/02)
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