- Fast and efficient extraction of DNA from meat and meat derived products using aqueous ionic liquid buffer systems
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A short, simple and inexpensive process for the extraction of DNA from meat was developed investigating a set of 20 ionic liquids including imidazolium, choline and guanidinium derivatives in combination with aqueous buffer systems. The environmentally benign ionic liquid choline hexanoate-phosphate buffer mixture gave the most promising results and DNA was extracted within 20 minutes from different types of meats, such as beef, chicken, pork and horse in significantly higher yields compared to the pure phosphate buffer. The influence of the ionic liquid on the amplification process during the PCR was further investigated, showing an inhibitory effect with increasing chain length of the ionic liquid and with higher ionic liquid concentrations. Additionally, extracted DNA was stable for 20 days when stored at room temperature in aqueous ionic liquid-buffer mixtures.
- Ressmann, Anna K.,García, Eric González,Khlan, Diana,Gaertner, Peter,Mach, Robert L.,Krska, Rudolf,Brunner, Kurt,Bica, Katharina
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supporting information
p. 4994 - 5002
(2015/06/16)
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- A green and sustainable approach to utilize bio-ionic liquids for the selective precipitation of high purity agarose from an agarophyte extract
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A few choline based bio-ionic liquids (bio-ILs) were employed for the first time for the selective precipitation of agarose from the hot seaweed extract of Gracilaria dura (an agarophyte) under ambient conditions. The method thus developed is much greener and economical in comparison with the methods widely practiced for agarose production. Among the bio-ILs, choline laurate was found to be the most effective for the isolation of agarose with a lower usage level (4.0%, w/w) with the yield of 14.0 ± 0.5% w/w. Agarose obtained by this process had the desired properties required for molecular biological applications and gel electrophoresis. Furthermore the bio-ILs were recycled and reused for subsequent batches of agarose isolation without compromising the yield and quality of biopolymers.
- Sharma, Mukesh,Prakash Chaudhary, Jai,Mondal, Dibyendu,Meena, Ramavatar,Prasad, Kamalesh
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p. 2867 - 2873
(2015/05/27)
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- Novel biocompatible cholinium-based ionic liquids - Toxicity and biodegradability
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The synthesis, characterisation and toxicological assessment of a new group of environmentally friendly ionic liquids are presented. Focussing on the toxic effect of the anion, the ionic liquids were designed by combining the benign cholinium cation, [NMe3(CH2CH2OH)]+, with a range of linear alkanoate anions ([CnH2n+1CO 2]-, n = 1-9), as well as two structural isomers (n = 3 or 4). The toxicity of these ionic liquids was evaluated using filamentous fungi as model eukaryotic organisms. Surprisingly, most of the tested species showed active growth in media containing extremely high ionic liquid concentrations, up to molar ranges in some cases. The biodegradability of these ionic liquids was assessed, and new biotechnological applications for them are proposed, e.g. as solvents for biopolymers. This study leads to the better understanding of the anion influence on the ionic liquid toxicity, but its core is the recognition that conscious design of ionic liquids can be used to deliver truly biocompatible salts without adversely affecting one of the most striking of their properties - their outstanding solvent ability.
- Petkovic, Marija,Ferguson, Jamie L.,Gunaratne, H. Q. Nimal,Ferreira, Rui,Leitao, Maria C.,Seddon, Kenneth R.,Rebelo, Luis Paulo N.,Pereira, Cristina Silva
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body text
p. 643 - 649
(2010/08/20)
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