Chemiluminescence quenching of pteroic acid-N-sulfonyl-acridinium-9- carboxamide conjugates by folate binding protein
N10-Trifluoroacetylpteroic acid was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamide labels at the N10 or 9-position carboxamide. Upon binding to folate binding protein the light output of the N10 derivative (9) was quenched up to 62% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (7) was quenched only 12%. The utility of this effect was demonstrated in a model homogeneous chemiluminescent assay for folic acid.
Adamczyk, Maciej,Fino, James R.,Mattingly, Phillip G.,Moore, Jeffrey A.,Pan, You
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