- Model Studies on Carboxypeptidase Y Catalyzed Peptide Synthesis in an Aqueous-Organic Two-Phase System
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Carboxypeptidase Y catalyzes in a biphasic system containing carbon tetrachloride and carbonate buffer the reaction of Z-Phe-OMe and various Z- and Boc-protected dipeptide methyl esters with Val-NH2 and Leu-NH2 respectively.This method has been applied to
- Kuhl, Peter,Zapevalova, Nina P.,Koennecke, Andreas,Jakubke, Hans-Dieter
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p. 343 - 348
(2007/10/02)
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- α-Chymotrypsin-catalyzed Synthesis of Tripeptide Amides in an Aqueous-Organic Two-phase System
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α-Chymotrypsin catalyzes in a biphasic system containing an organic solvent and hydrogencarbonate buffer the reaction of Boc-Leu-Phe-OMe with X-NH2 (X=Leu, Met, Val) forming Boc-tripeptide amides.In the case of X=Leu the addition of ammoniumsulfate is advantageous and allows the use of carboxyl and amino component in equivalent amounts.By the same method Z-protected tripeptide amides can be prepared from Z-X-Phe-OMe (X=Ala, Leu, Val) and Leu-NH2 in good yields.In some case α-chymotrypsin-catalyzed peptide coupling is also possible, if the buffer solution is substituted for crystal water containing sodium carbonate.
- Kuhl, P.,Posselt, Siegrid,Jakubke, H.-D.
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p. 463 - 465
(2007/10/02)
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