- Optimised N-acetyl-D-lactosamine synthesis using Thermus thermophilus β-galactosidase in bio-solvents
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Synthesis of N-acetyl-D-lactosamine (Gal-β[1→4]GlcNAc, LacNAc) catalyzed by β-galactosidase from Thermus thermophilus (TTP0042) is affected by side reactions that give as result very low yields (about 20%) of LAcNAc when the reaction is performed in buffer. The process is improved (up to 91% of disaccharide yield) when the reaction takes place in the presence of solvents from biomass (bio-solvents) at 2.0 M concentration. Most of the solvents tested increased the LacNAc synthesis and reduced the undesired side reactions. In order to understand the possible effects of these solvents over the enzyme regioselectivity, we developed a conformational study of the enzyme structure in the presence of a selected bio-solvent by circular dichroism and fluorescence. According to this study, we were able to conclude that the presence of bio-solvents in the reaction media modifies the enzyme secondary and tertiary structure and this may be the cause of the regioselectivity changes observed in the transglycosylation reaction.
- Sandoval, Manuel,Civera, Concepción,Berenguer, José,García-Blanco, Francisco,Hernaiz, María J.
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p. 1148 - 1152
(2013/02/25)
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- Creation of an α-mannosynthase from a broad glycosidase scaffold
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α-Mannosides made easy: Mutation of a family-GH31 α-glucosidase that displays plasticity to alterations at the 2-OH position of donor substrates created an efficient α-mannoside-synthesizing biocatalyst. A simple fluoride donor reagent was used for the synthesis of a range of mono-α-mannosylated conjugates using the α-mannosynthase displaying low (unwanted) oligomerization activity. Copyright
- Yamamoto, Keisuke,Davis, Benjamin G.
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supporting information; experimental part
p. 7449 - 7453
(2012/09/21)
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- Acceptor-dependent regioselectivity of glycosynthase reactions by Streptomyces E383A β-glucosidase
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The nonnucleophilic mutant E383A β-glucosidase from Streptomyces sp. has proven to be an efficient glycosynthase enzyme, catalyzing the condensation of α-glucosyl and α-galactosyl fluoride donors to a variety of acceptors. The enzyme has maximal activity
- Faijes, Magda,Saura-Valls, Marc,Perez, Xavi,Conti, Marta,Planas, Antoni
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p. 2055 - 2065
(2007/10/03)
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- Chemoenzymatic synthesis of spacer-linked oligosaccharides for the preparation of neoglycoproteins
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In the present work, the combination of chemical and enzymatic methods to obtain neoglycoproteins is described. Three bovine serum albumin (BSA)-conjugates, BSA-[GalNAcα-], BSA-[Gal(β1-3)GalNAc(α-], and BSA-[Neu5Ac(α2-3)Gal(β1-3)GalNAc(α-], were prepared.
- Gonzalez Lio, Raul,Thiem, Joachim
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p. 180 - 190
(2007/10/03)
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- p-NITROPHENYL 2-, AND 3-O-α-D-MANNOPYRANOSYL-α-D-MANNOPYRANOSIDE
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p-Nitrophenyl 3- and 2-O-benzoyl-4,6-O-benzylidene-α-D-mannopyranoside were each condensed with 2,3,4,6-tetra-O-benzoyl-α-D-mannopyranosyl bromide, and the products were deprotected, to yield, respectively, p-nitrophenyl 2- and 3-O-mannopyranosyl-α-D-mann
- Ekborg, Goeran,Glaudemans, Cornelis P. J.
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- SYNTHESIS OF p-NITROPHENYL 3-O-β-D-GALACTOPYRANOSYL-β-D-GALACTOPYRANOSIDE AND p-NITROPHENYL 3-O-α-D-GALACTOPYRANOSYL-β-D-GALACTOPYRANOSIDE
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Glycosylation (catalyzed by mercuric cyanide) of p-nitrophenyl 2,4,6,-tri-O-acetyl-β-D-galactopyranoside (2) with 2,3,4,6-tetra-O-acetyl-α-D-galactopyranosyl bromide in acetonitrile afforded the α-(1->3)- and β-(1->3)-linked disaccharide heptaacetates (4
- Abbas, Saled A.,Barlow, Joseph J.,Matta, Khushi L.
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p. 231 - 244
(2007/10/02)
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