Practical Synthesis of 5-Phospho-D-ribosyl α-1-pyrophosphate (PRPP): Enzymatic Routes from Ribose 5-Phosphate or Ribose
This paper describes enzymatic syntheses of 5-phospho-D-ribosyl α-1-pyrophosphate (PRPP) on a 75-mmol scale.The reactions used PAN-immobilized PRPP synthetase as catalyst with in situ ATP-cofactor regeneration.In one procedure pure r-5-P was used as a starting material; in a second, r-5-P was synthesized by ribokinase-catalyzed phosphorilation of D-ribose and used in situ.The potential for use of PRPP as a starting material for the preparation of nucleotides was demonstrated by an enzymatic synthesis of UMP.This paper also describes several methods for the preparation of r-5-P: acid-catalyzed hydrolysis of AMP, acid-catalyzed hydrolysis of crude mononucleotide mixture obtained by digestion of RNA, chemical synthesis from D-ribose, and ribokinase-catalyzed synthesis from D-ribose.Procedures are described for the isolation of PRPP synthetase (from Salmonella typhimurium) and ribokinase (from Lactobacillus plantarum) and for immobilization of these enzymes in PAN.
Gross, Akiva,Abril, Obsidiana,Lewis Jerome M.,Geresh, Shimona,Whitesides, George M.
p. 7428 - 7435
(2007/10/02)
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