- A Potentially Useful Fluorogenic Amine, 4-Amino-7-nitrobenz-2-oxa-1,3-diazole. An Application as a Substrate for a Microdetermination of Chymotrypsin
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4-Amino-7-nitrobenz-2-oxa-1,3-diazole (1b) is a potentially useful key fluorogenic amine.As an example of the application of this amine, 7-(N-tosyl- and N-mesyl-L-phenylalanyl)amino-4-nitrobenz-2-oxa-1,3-diazole (2) were prepared and shown to be good fluorogenic substrates for the assay of chymotrypsin.Keywords - fluorometric enzyme assay; fluorogenic amine; 4-amino-7-nitrobenz-2-oxa-1,3-diazole; 7-(N-tosyl-L-phenylalanyl)amino-4-nitrobenz-2-oxa-1,3-diazole; 7-(N-mesyl-L-phenylalanyl)amino-4-nitrobenz-2-oxa-1,3-diazole; kinetic study
- Sato, Eisuke,Miyakawa, Makiko,Kanaoka, Yuichi
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p. 336 - 339
(2007/10/02)
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