Preparation of New Haptens for Use in Immunoassay of Glycine-Conjugated Bile Acids
The N-succinimidyl esters of various glycine-conjugated bile acid 3-hemisuccinates and 3-hemiglutarates have been prepared for use in immunoassay.Unconjugated bile acids were condensed with p-nitrophenol to form the activated esters.Subsequent reaction wi