- Probing the catalytic promiscuity of a regio- and stereospecific C-glycosyltransferase from Mangifera indica
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The catalytic promiscuity of the novel benzophenone C-glycosyltransferase, MiCGT, which is involved in the biosynthesis of mangiferin from Mangifera indica, was explored. MiCGT exhibited a robust capability to regio- and stereospecific C-glycosylation of 35 structurally diverse druglike scaffolds and simple phenolics with UDP-glucose, and also formed O- and N-glycosides. Moreover, MiCGT was able to generate C-xylosides with UDP-xylose. The OGT-reversibility of MiCGT was also exploited to generate C-glucosides with simple sugar donor. Three aryl-C-glycosides exhibited potent SGLT2 inhibitory activities with IC50 values of 2.6×, 7.6×, and 7.6×10-7-M, respectively. These findings demonstrate for the first time the significant potential of an enzymatic approach to diversification through C-glycosidation of bioactive natural and unnatural products in drug discovery. C-glycodiversification: MiCGT, as the first benzophenone C-glycosyltransferase (CGT) from Mangifera indica, showed robust regio- and stereospecific C-glycosylation activity for 35 structurally diverse acceptors with UDP-glucose or xylose. The aryl-C-glycoside 1 exhibited potent antidiabetic activity toward SGLT2.
- Chen, Dawei,Chen, Ridao,Wang, Ruishan,Li, Jianhua,Xie, Kebo,Bian, Chuancai,Sun, Lili,Zhang, Xiaolin,Liu, Jimei,Yang, Lin,Ye, Fei,Yu, Xiaoming,Dai, Jungui
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supporting information
p. 12678 - 12682
(2015/10/28)
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- DEOXYGENATION OF ALDEHYDES AND KETONES WITH SODIUM CYANOBOROHYDRIDE
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Treatment of hydroxy-substituted aromatic aldehydes and ketones with sodium cyanoborohydride yields the corresponding methylene compounds under conditions which favor intermediate carbonium ion formation.
- Elliger, Carl A.
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p. 1315 - 1324
(2007/10/02)
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- Enol Ethers, XIV. Acylation of Keto Enol Ethers with Malonyl Dichloride - A New Synthesis of Phloroglucinols
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Phloroglucinols 4 and/or 4-hydroxy-2H-pyran-2-ones 5 are formed from keto enol ethers 1 and malonyl dichloride (2a) in high yields.Since the pyranones 5 can be smoothly converted into phloroglucinols, the reaction of 1 with 2a represents a new, facile synthetic route to phloroglucinols.The reaction proceeds via formation of a chloro carbonyl ketene 8 and its subsequent reaction with 1.The product ratio 4:5 is rationalized in terms of substituent effects in the enol ether substrate.
- Effenberger, Franz,Schoenwaelder, Karl-Heinz
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p. 3270 - 3279
(2007/10/02)
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- CONDENSED TANNINS: COMPETING NUCLEOPHILIC CENTRES IN BIOMIMETIC CONDENSATION REACTIONS
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The relative contributions of nucleophilicity and steric hindrance in determining the course of the reacition during the formation of 'angular' triflavonoids are examined by biomimetic-type condensations involving model compounds.Key Word Index - (4,6)-(-)-fisetinidol-8-(4-hydroxybenzyl)-(+)-catechins; 4-(3-ethyl-2,4,6-trihydroxyphenyl)-3,3',4',7-tetrahydroxyflavans; (4,3:4,5)-bi--ethyl phloroglucinols; condensed tannins; nucleophilicity; steric hindrance.
- Botha, Jacobus J.,Viviers, Phillip M.,Ferreira, Daneel,Roux, David G.
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p. 1289 - 1294
(2007/10/02)
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