10257-28-0Relevant articles and documents
QUERCETIN 3-O-GALACTOSYL-(1 -> 6)-GLUCOSIDE, A COMPOUND FROM NARROWLEAF WETCH WITH ANTIBACTERIAL ACTIVITY
Waage, Susan K.,Hedin, Paul A.
, p. 243 - 246 (1985)
A new flavonol glycoside, quercetin 3-O-galactosyl-(1 -> 6)-glucoside, has been isolated from above-ground parts of narrowleaf vetch, Vicia angustifolia.Its antibacterial activity against Pseudomonas maltophilia and Enterobacter cloacae is compared with that of several other flavonol glycosides.Key Word Index - Vicia angustifolia; Leguminosae; flavonol glycoside; quercetin; antibacterial.
Synthesis of some trifluoromethylated cyclodextrin derivatives and analysis of their properties as artificial glycosidases and oxidases
Bjerre, Jeannette,Fenger, Thomas Hauch,Marinescu, Lavinia G.,Bols, Mikael
, p. 704 - 710 (2007)
Cyclodextrin derivatives containing trifluoromethyl groups at C6 of the A and D rings were synthesized for the purpose of creating artificial enzymes. The compounds were synthesized by perbenzylation of β-cyclodextrin followed by selective A,D-debenzylation according to Sinay. Subsequent oxidation to dialdehyde with Dess-Martin periodinane followed by addition of CF3 by using Arduengo carbene and TMSCF3 led to the C6- bistrifluoromethylated alcohols. These were either deprotected by hydrogenolysis or subjected to another round of oxidation to provide the corresponding ketones that were deprotected. The trifluoromethylated alcohols were found to be weak artificial enzymes catalysing hydrolysis of nitrophenyl glycosides at neutral pH with a kcat/kuncat of up to 56. It is proposed that this catalysis is analogues to the catalysis performed by related cyanohydrins. The trifluoro ketones were likewise weak articial enzymes catalysing oxidation of amines to nitro derivatives or alcohols to ketones with a kcat/k uncat of up to 133. Wiley-VCH Verlag GmbH & Co. KGaA, 2007.
Comparative study of new α-galactosidases in transglycosylation reactions
Spangenberg, Petra,Andre, Corinne,Dion, Michel,Rabiller, Claude,Mattes, Ralf
, p. 65 - 73 (2000)
We have studied the potential of several newly cloned α-galactosidases to catalyze the regioselective synthesis of disaccharides using 4-nitrophenylgalactoside as a donor. The kinetics of the reactions were followed by in situ NMR spectroscopy. The following thermophilic enzymes have been tested: Aga A and an isoenzyme Aga B obtained from the strain KVE39 and Aga 285 from the strain IT285 of Bacillus stearothermophilus; Aga T is an α-galactosidase from Thermus brockianus (strain IT360). Two other non-thermophilic α-galactosidases have also been evaluated: Aga 1 (Streptococcus mutans, strain Ingbritt) and Raf A (Escherichia coli, strain D1021). For all of the enzymes studied, high regioselectivity was observed leading to two (1 → 6)-disaccharides: 4-nitrophenyl α-D-galactopyranosyl-(1 → 6)-α-D-galactopyranoside and methyl α-D-galactopyranosyl-(1 → 6)-α-D-galactopyranoside, which were obtained in 54% (Aga B) and 20% (Aga T) yields, respectively. (C) 2000 Elsevier Science Ltd.
Two new triterpene saponins from Acanthophyllum laxiusculum
Pertuit, David,Baghery Lotfabad, Tayebe,Mitaine-Offer, Anne-Claire,Miyamoto, Tomofumi,Tanaka, Chiaki,Lacaille-Dubois, Marie-Aleth
, p. 611 - 617 (2015)
Two new triterpene glycosides, 1 and 2, together with three known ones, were isolated from roots of Acanthophyllum laxiusculum Schiman-Czeika. The structures of the new compounds were established by extensive 1D- and 2D-NMR spectroscopic experiments and MS analyses as 23-O-β-D-galactopyranosylgypsogenic acid 28-O-{β-D-glucopyranosyl-(1→2)-6-O-[4-carboxy-3-hydroxy-3-methyl-1-oxobutyl]-β-D-glucopyranosyl-(1→6)}-[β-D-glucopyranosyl-(1→3)]-β-D-galactopyranosyl ester (1) and gypsogenic acid 28-O-{β-D-glucopyranosyl-(1→2)-6-O-[4-carboxy-3-hydroxy-3-methyl-1-oxobutyl]-β-D-glucopyranosyl-(1→6)}-[β-D-glucopyranosyl-(1→3)]-β-D-galactopyranosyl ester (2).
Remarkable supramolecular catalysis of glycoside hydrolysis by a cyclodextrin cyanohydrin
Ortega-Caballero, Fernando,Rousseau, Cyril,Christensen, Brian,Petersen, Torben Ellebaek,Bols, Mikael
, p. 3238 - 3239 (2005)
(6AR,6DR)-6A,6D-di-C-cyano-β-cyclodextrin (3) was synthesized and shown to catalyze hydrolysis of nitrophenyl glycosides with the reaction following Michaelis-Menten kinetics. At pH 7.4 and 25 °C, hydrolysis of 4-nitrophenyl-β-glucopyranoside (2) was catalyzed with KM = 15 mM, kcat = 8.2 × 10-6 s-1, and kcat/kuncat = 1217. Catalysis was observed with concentration of 3 as low as 10 μM. Hydrolysis of the corresponding α-glucoside, α-galactoside, α-mannoside, and 2-nitrophenyl-β-galactoside was also catalyzed by 3, with kcat/kuncat ranging from 283 to 2147. A series of analogues of 3 was prepared and investigated for catalysis of the hydrolysis of 2: (6AR,6DR)-6A,6D-di-C-propyl-β-cyclodextrin (9) was not catalytic, while 6A,6D-di-C-cyano-6A,6D-dideoxy-β-cyclodextrin (12) had a low catalytic activity (kcat/kuncat = 4). A kcatkuncat = 48 was found for 6A,6D-dialdehydo-β-cyclodextrin dihydrate (11). It was proposed that 3 acts by general acid catalysis on the bound substrate. Copyright
Structures of steroidal alkaloid oligoglycosides, robeneosides A and B, and antidiabetogenic constituents from the Brazilian medicinal plant Solarium lycocarpum
Yoshikawa, Masayuki,Nakamura, Seikou,Ozaki, Kenichi,Kumahara, Akira,Morikawa, Toshio,Matsuda, Hisashi
, p. 210 - 214 (2007)
In the course of our studies on antidiabetogenic compounds from natural medicines and medicinal foodstuffs, a methanolic extract of the fruits of Solanum lycocarpum showed an inhibitory effect on the increase of serum glucose levels in oral sucrose-loaded rats. Through bioassay-guided separation, three known steroidal alkaloid oligoglycosides, solamargine (1), solasonine (3), and 12-hydroxysolasonine (5), were isolated from the active fraction together with two new steroidal alkaloid oligoglycosides, robeneosides A (2) and B (4). The major constituents, solamargine (1) and solasonine (3), exhibited an inhibitory effect on the increase of serum glucose levels in oral sucrose-loaded rats. In addition, these compounds suppressed gastric emptying in mice. However, this methanolic extract from S. lycocarpum fruits did not have any effect on intestinal a-glucosidase (sucrase and maltase) in vitro. It was concluded that these steroidal alkaloid oligoglycosides, 1 and 3, inhibited the increase of rat serum glucose levels by suppressing the transfer of sucrose from the stomach to the small intestine.
Illuminating the binding interactions of galactonoamidines during the inhibition of β-galactosidase (E. coli)
Fan, Qiu-Hua,Pickens, Jessica B.,Striegler, Susanne,Gervaise, Cédric D.
, p. 661 - 671 (2016)
Several galactonoamidines were previously identified as very potent competitive inhibitors that exhibit stabilizing hydrophobic interactions of the aglycon in the active site of β-galactosidase (Aspergillus oryzae). To elucidate the contributions of the glycon to the overall inhibition ability of the compounds, three glyconoamidine derivatives with alteration in the glycon at C-2 and C-4 were synthesized and evaluated herein. All amidines are competitive inhibitors of β-galactosidase (Escherichia coli) and show significantly reduced inhibition ability when compared to the parent. The results highlight strong hydrogen-bonding interactions between the hydroxyl group at C-2 of the amidine glycon and the active site of the enzyme. Slightly weaker H-bonds are promoted through the hydroxyl group at C-4. The inhibition constants were determined to be picomolar for the parent galactonoamidine, and nanomolar for the designed derivatives rendering all glyconoamidines very potent inhibitors of glycosidases albeit the derivatized amidines show up to 700-fold lower inhibition activity than the parent.
Oleanane glycosides from the roots of Alhagi maurorum
Hamed, Arafa,Perrone, Angela,Mahalel, Usama,Oleszek, Wieslaw,Stochmal, Anna,Piacente, Sonia
, p. 782 - 787 (2012)
Three new oleanane-type triterpene glycosides (1-3), along with four known compounds (4-7) glycosides, were isolated from the roots of Alhagi maurorum. Their structures were elucidated by 1D and 2D-NMR experiments as well as ESI-MS analysis. The antiproliferative activity of the isolated compounds was evaluated against a small panel of cancer cell lines including human breast cancer (MCF-7), human lung adenocarcinoma (A549), human prostate cancer (PC-3) and human leukemia (U937) cell lines. None of the tested compounds, in a range of concentrations between 1 and 50 μM, caused a significant reduction of the cell number.
Two new acylated flavonol glycosides from the seeds of Lepidium sativum
Fan, Qing-Lu,Zhu, Yin-Di,Huang, Wen-Hua,Qi, Yun,Guo, Bao-Lin
, p. 11341 - 11349 (2014)
Two new acylated flavonol glycosides named kaempferol-3-O-(2-O-sinapoyl)- β-D-galactopyranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L- rhamnopyranoside (1) and quercetin-3-O-(6-O-benzoyl)-β-D-glucopyranosyl- (1→3)-β-D-galactopyranoside-7-O-α-L-rhamnopyranoside
Flavonol glycosides with α-D-aldohexoses from Rhododendron irroratum
Yang, Ming Hua,Luo, Jian Guang,Huang, Xue Feng,Kong, Ling Yi
, p. 920 - 925 (2010)
Two new flavonol glycosides which contain rare α-D-galactose or α-D-glucose were obtained from the flowers of Rhododendron irroratum Franch., namely myricetin 3-O-β-D-galactoside-3′-O -α-D-glucoside (1) and myricetin 3-O-β-D-galactoside-3′-O- α-D-glucosid
Karataviosides G-K, five new bisdesmosidic steroidal glycosides from the bulbs of Allium karataviense
Kuroda, Minpei,Ori, Kazutomo,Takayama, Hiroshi,Sakagami, Hiroshi,Mimaki, Yoshihiro
, p. 96 - 104 (2015)
We have analyzed the steroidal glycosides in Allium karataviense bulbs, and isolated five new bisdesmosidic steroidal glycosides: karataviosides G-K (1-5). The structures were elucidated by extensive spectroscopic analysis, including 2D NMR and enzymatic and hydrolytic cleavage. Karatavioside G (1) is an entirely novel furostanol glycoside, which has an O-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl unit at C-26 of the aglycone. Although a variety of cholestanol glycosides have been isolated, mainly from Liliaceae and Agavaceae, karataviosides J and K (4 and 5) are also notable because they are the most polar cholestanol bisdesmosides discovered, in which a lycotetraose is attached to C-3 of the aglycone, and a glucose or O-glucosyl-(1 → 3)-glucose is attached at C-16. The isolated glycosides were also evaluated for their cytotoxic activities against cultured tumor cell lines.
β-GALACTOSIDASE FROM SUGAR CANE
Etcheberrigaray, Juan Lorenzo,Vattuone, Marta Amelia,Sampietro, Antonio Rodolfo
, p. 49 - 52 (1981)
Key Word Index - Saccharum officinarium; Gramineae; sugar cane; β-galactosidase; cell wall. β-Galactosidase activity occurs in all of the organs of the sugar cane plant, and is also of general occurrence among different cultivars and species.Most of the activity was associated with the cell wall, and only ca 12-16 percent was an intercellular form.Both activities posess similar optimum pH and Km, both are activated by Mn2+ and ethanol, and inhibited by Hg2+, and both attack the same substrates.
Structural characterization of a new steroidal saponin from Agave angustifolia var. Marginata and a preliminary investigation of its in vivo antiulcerogenic activity and in vitro membrane permeability property
Pereira, Gabriela Moysés,Ribeiro, Marcela Gon?alves,da Silva, Bernadete Pereira,Parente, José Paz
, p. 4345 - 4349 (2017)
A new furostane steroidal saponin was isolated from the leaves of Agave angustifolia var. marginata. On the basis of chemical conversions and spectroscopic analyses, its structure was established as 3-[O-β-D-glucopyranosyl-(1→3)-O-β-D-glucopyranosyl-(1→3)-O]-[O-6-deoxy-α-L-mannopyranosyl-(1→4)-β-D-xylopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-(3β,5α,22α,25R)-26-(β-D-glucopyranosyloxy)-22-methoxy-furostane (1). Results of preliminary biological investigations indicated that compound 1 showed significant protective effects against induced gastric ulcers using in vivo experimental models and demonstrated negligible toxicity on membrane integrity in the in vitro assays.
Isolation, characterization and anti-inflammatory property of Thevetia Peruviana
Thilagavathi,Kavitha, Helen P.,Venkatraman
, p. 1584 - 1590 (2010)
Thevetia peruviana seeds contain glucosides of neriifolin, acetylneriifolin and thevetin. Seed oil distillates of Thevetia peruviana have been found to contain anti-bacterial activity. In the persent work, the fresh flowers of Thevetia peruviana was subjected to phytochemical studies. The results of the study showed that the flowers contain quercetin, kaempferol and quercetin-7-o-galactoside. The structure of the isolated compound was characterized by UV, 1H NMR and 13C NMR spectra. The anti-inflammatory character of the isolated compound was tested by in vitro method and the results of the study revealed that the isolated compound showed a biphasic property.
Effect of magnesium cations on the activity and stability of β-galactosidases
Atyaksheva,Pilipenko,Poltorak,Chukhrai
, p. 1156 - 1159 (2007)
It was shown that the presence of magnesium cations in the reaction mixture increases, approximately twofold, the activity of bacterial Escherichia coli and yeast Kluyveromyces lactis β-galactosidases but does not affect the activity of bovine liver and f
Synthesis and biological evaluation of a novel MUC1 glycopeptide conjugate vaccine candidate comprising a 4'-deoxy-4'-fluoro-Thomsen-Friedenreich epitope
Johannes, Manuel,Reindl, Maximilian,Gerlitzki, Bastian,Schmitt, Edgar,Hoffmann-R?der, Anja
, p. 155 - 161 (2015)
The development of selective anticancer vaccines that provide enhanced protection against tumor recurrence and metastasis has been the subject of intense research in the scientific community. The tumor-associated glycoprotein MUC1 represents a well-established target for cancer immunotherapy and has been used for the construction of various synthetic vaccine candidates. However, many of these vaccine prototypes suffer from an inherent low immunogenicity and are susceptible to rapid in vivo degradation. To overcome these drawbacks, novel fluorinated MUC1 glycopeptide-BSA/TTox conjugate vaccines have been prepared. Immunization of mice with the 4'F-TF-MUC1-TTox conjugate resulted in strong immune responses overriding the natural tolerance against MUC1 and producing selective IgG antibodies that are cross-reactive with native MUC1 epitopes on MCF-7 human cancer cells.
New triterpene saponins from the roots of Acacia macrostachya (Mimosaceae)
Tchoukoua, Abdou,Tabopda, Turibio Kuiate,Usukhbayar, Narandulam,Kimura, Ken-ichi,Kwon, Eunsang,Momma, Hiroyuki,Koseki, Takuya,Shiono, Yoshihito,Ngadjui, Bonaventure Tchaleu
, p. 2261 - 2267 (2017)
Four new oleanane-type saponins, macrostachyaosides A, B, C, and D (1–4) were isolated from the roots of Acacia macrostachya. Their structures were elucidated on the basis of extensive 1D- and 2D-NMR data and HR-ESI-MS analyses. At concentrations of 100 μM of each compounds, none of the tested compounds caused a significant growth reduction against HL60 cells.
The phenolic acids from Oplopanax elatus Nakai stems and their potential photo-damage prevention activity
Han, Yu,Cheng, Dongsheng,Hao, Mimi,Yan, Jiejing,Ruan, Jingya,Han, Lifeng,Zhang, Yi,Wang, Tao
, p. 39 - 48 (2022)
25 phenolic acids, including four new isolates, eurylophenosides A–D (1–4) and 21 known ones (5–25) were isolated and identified from the stems of Oplopanax elatus Nakai. Among the known compounds 5–9, 11–13, 16, 18–25 were isolated from the genus for the first time; 17 was first obtained from the plant; and the NMR data of 22 was reported here first. Meanwhile, the UVB-induced photodamage model of HaCaT cells was used to study the prevent-photodamage abilities of compounds 1–2, 4–8, 11–13 and 15–25 with a nontoxic concentration at 50?μM. Moreover, a dose-dependent experiment was conducted for active compounds at the concentration of 10, 25, and 50?μM, respectively. Consequently, pretreatment with compounds 1, 16, 17, 19, 20, 22, 24 and 25 could suppress the cell viability decreasing induced by UVB irradiation in a concentration-dependent manner. These results indicated that phenolic acids were one kind of material basis with prevent-photodamage activity of O. elatus. Graphic abstract: [Figure not available: see fulltext.].
Kaempferol and its glycosides from Equisetum silvaticum L. from the khanty-mansi autonomous area
Bonacheva,Botirov, E. Kh.
, p. 777 - 780 (2014)
Three flavonoids were isolated from the aerial part of the wood horsetail (Equisetum silvaticum L.); two of them were found for the first time. The compounds were identified as kaempferol, kaempferol 3-O-β-D-galactopyranosyl-7-O-α-L-rhamnopyranoside and kaempferol 3-O-rutinosyl-7-O-L-rhamnopyranoside on the basis of the chemical transformations and IR, UV, 1H-NMR and mass spectra.
Antifungal saponins from bulbs of garlic, Allium sativum L. var. Voghiera
Lanzotti, Virginia,Barile, Elisa,Antignani, Vincenzo,Bonanomi, Giuliano,Scala, Felice
, p. 126 - 134 (2012)
A bioassay-guided phytochemical analysis of the polar extract from the bulbs of garlic, Allium sativum L., var. Voghiera, typical of Voghiera, Ferrara (Italy), allowed the isolation of ten furostanol saponins; voghieroside A1/A2 and voghieroside B1/B2, based on the rare agapanthagenin aglycone; voghieroside C1/C2, based on agigenin aglycone; and voghieroside D1/D2 and E1/E2, based on gitogenin aglycone. In addition, we found two known spirostanol saponins, agigenin 3-O-trisaccharide and gitogenin 3-O-tetrasaccharide. The chemical structures of the isolated compounds were established through a combination of extensive nuclear magnetic resonance, mass spectrometry and chemical analyses. High concentrations of two eugenol diglycosides were also found for the first time in Allium spp. The isolated compounds were evaluated for their antimicrobial activity towards two fungal species, the air-borne pathogen Botrytis cinerea and the antagonistic fungus Trichoderma harzianum.
Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L. (Ranunculaceae)
Mariani, Cristina,Braca, Alessandra,Vitalini, Sara,De Tommasi, Nunziatina,Visioli, Francesco,Fico, Gelsomina
, p. 1220 - 1226 (2008)
In this paper, we report studies on morphological, phytochemical, and biological aspects of a population belonging to Aconitum anthora L. Two compounds, quercetin 3-O-((β-d-glucopyranosyl-(1→3)-(4-O-(E-p-coumaroyl))-α-l -rhamnopyranosyl-(1→6)-β-d-galactopyranoside))-7-O-α-l-r hamnopyranoside (1) and kaempferol 3-O-((β-d-glucopyranosyl-(1→3)-(4-O-(E-p-coumaroyl))-α-l -rhamnopyranosyl-(1→6)-β-d-galactopyranoside))-7-O-α-l-r hamnopyranoside (2), together with two known flavonol glycosides (3-4) were isolated and identified from A. anthora. The antioxidant activity of the four identified flavonoids was screened by three in vitro tests.
Anderson,Karamalla
, p. 762 (1966)
New Steroidal Saponins from the Leaves of Yucca elephantipes
Zhang, Ying,Yang, Chong-Ren,Zhang, Ying-Jun
, p. 1807 - 1813 (2013)
Two new spirostanol saponins, namely elephanosides G and H (1 and 2, resp.) were isolated from the leaves of Yucca elephantipes (Agavaceae), together with the two known furostanol saponins 3 and 4 and the six known flavonoid O- and C-glycosides 5-10. The
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Bianco,A.,Guiso,M.
, p. 465 (1981)
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Antiproliferative steroidal glycosides from Digitalis ciliata
Perrone, Angela,Capasso, Anna,Festa, Michela,Kemertelidze, Ether,Pizza, Cosimo,Skhirtladze, Alexandre,Piacente, Sonia
, p. 554 - 562 (2012)
Two new compounds, a furostanol glycoside (1) and a pregnane glycoside (4), along with eight known compounds, belonging to the classes of spirostane (2,3), pregnane (5-7) and cardenolide (8-10) glycosides, were isolated from the seeds of Digitalis ciliata. Their structures were elucidated by 1D and 2D-NMR experiments as well as ESI-MS analysis. For the first time pregnane glycosides of the diginigenin series have been isolated from D. ciliata. The cytotoxic effects of compounds 1-10 on cell viability of several cancer cell lines, namely human breast cancer (MCF-7), human glioblastoma (T98G), human lung adenocarcinoma (A549), human colon carcinoma (HT-29), and human prostate cancer (PC-3) cell lines were evaluated. Compounds 1, 4, 7 and 8 showed antiproliferative effects against MCF-7, HT-29 and A549 cancer cells with IC50 values ranging from 8.3 to 20 μM. The effects of compounds 1-10 on cell proliferation were evaluated on these three cancer cell lines by cell cycle analysis of DNA content using flow cytometry. Compounds 7, 8 and 10 induced significant changes in G2/M cell cycle phase of all analyzed cells. The obtained results indicate that compounds 7, 8 and 10 are cytostatic compounds effective in reducing cell proliferation by inducing accumulation of the cells in the G2/M phase of the cell cycle.
Polythosides A and B, two new triterpenoid saponins from the roots of Acacia polyacantha Willd. (Mimosaceae)
Tchoukoua, Abdou,Tomfeun Nganou, Sandrine Carole,Dabole, Bernard,Yoshida, Jun,Ito, Yoshiaki,Ngadjui, Bonaventure Tchaleu,Shiono, Yoshihito
, p. 190 - 195 (2021/05/03)
Two new oleanane-type triterpene saponins, named polythosides A and B (1 and 2), together with a known compound, silphioside E (3), were isolated from the roots of Acacia polyacantha. Their structures were elucidated by analysis of 1D and 2D-NMR experiments, and mass spectrometry (HR-ESITOF-MS) as oleanolic acid 3-O-β-D-galactopyranosyl-(1→2)-β-D-xylopyranosyl-(1→6)-[β-D-xylopyranosyl-(1→4)]-[β-D-xylopyranosyl-(1→2)]-β-D-galactopyranoside (1), 3-O-[β-D-galactopyranosyl-(1→2)-β-D-xylopyranosyl-(1→6)-[β-D-xylopyranosyl-(1→4)]-[β-D-xylopyranosyl-(1→2)]-β-D-galactopyranosyl] oleanolic acid-28-O-β-D-glucopyranosyl ester (2) and 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl] oleanolic acid 28-O-β-D-glucopyranosyl ester (3). The cytotoxic effect of isolated saponins was evaluated on the H4IIE rat hepatoma cell line. The results show that none of the compounds (assayed at 100 μM) showed cytotoxicity against H4IIE cells.
Flavonoid glucosides from the flowers of Trollius chinensis Bunge
Yan, Rui,Cui, Yida,Deng, Bowen,Bi, Jiayi,Zhang, Guogang
, p. 297 - 302 (2018/11/27)
Four new flavonoid glucosides, 2″-O-(6?-O-caffeoyl)-galactopyranosylvitexin (1), 2″-O-(6?-O-veratroyl)-galactopyranosylvitexin (2), 2″-O-(6?-O-feruloyl)-galactopyranosylorientin (3), 2″-O-(2?-O-methylbutyryl)-glucopyranosylisoswertisin (4), along with three known compounds were isolated from the flowers of Trollius chinensis Bunge. Their structures were determined by extensive spectroscopic analyses.
