105728-02-7Relevant articles and documents
Hapten synthesis, monoclonal antibody production and immunoassay development for direct detection of 4-hydroxybenzehydrazide in chicken, the metabolite of nifuroxazide
Mari, Ghulam Mujtaba,Li, Hongfang,Dong, Baolei,Yang, Huijuan,Talpur, Aisha,Mi, Jiafei,Guo, Liuchuan,Yu, Xuezhi,Ke, Yuebin,Han, Diangang,Wang, Zhanhui
, (2021)
Derivatization is usually employed in immunoassay for detection of metabolites of nitrofurans and avoiding derivatization could be preferable to achieve an efficient screening. In the study, we designed four haptens of 4-hydroxybenhydrazide (HBH), the nifuroxazide metabolite. The effect of hapten structures on antibody affinity were evaluated and one monoclonal antibody was produced by using the Hapten C with a linear alkalane spacer arm. After optimization, an enzyme linked-immunosorbent assay (ELISA) was established with an 50% inhibition concentration of 0.25 ng mL?1 for HBH, which could ensure the direct detection of HBH without derivatization. The limit of detection of the ELISA for HBH was 0.12 μg kg?1 with the recoveries of 90.1–96.2% and coefficient of variation (CV) values lower than 9.1%. In conclusion, we produced several high affinity antibodies to HBH with new designed hapten and developed an icELISA for the direct detection of HBH without derivatization in chicken.
Nifuraldezone hapten and artificial antigen as well as preparation method and application thereof
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Paragraph 0075-0076; 0079-0080, (2020/02/06)
The invention relates to nifuraldezone hapten and artificial antigen as well as a preparation method and application thereof. The nifuraldezone hapten has a structural formula (I) or (II) shown in thedescription. The nifuraldezone artificial antigen is prepared by coupling the hapten of the formula (I) or (II) with a carrier protein. When the nifuraldezone artificial antigen is adopted to immunize animals, specific antibodies with high valences and high sensitivity can be obtained. By adopting the nifuraldezone hapten and antibodies prepared from same, novel means for establishing rapid, simple, cheap, sensitive and specific nifuraldezone detection methods can be provided.