109244-58-8 Usage
Description
DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M?1cm?1). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.
Uses
Different sources of media describe the Uses of 109244-58-8 differently. You can refer to the following data:
1. Dihydrorhodamine 123 is the uncharged and nonfluorescent reduction product of the mitochondrion-selective dye rhodamine 123 used for investigation of reacitve oxygen intermediates.
2. Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.
General Description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.
Biochem/physiol Actions
Cell permeable: yes
Check Digit Verification of cas no
The CAS Registry Mumber 109244-58-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,9,2,4 and 4 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 109244-58:
(8*1)+(7*0)+(6*9)+(5*2)+(4*4)+(3*4)+(2*5)+(1*8)=118
118 % 10 = 8
So 109244-58-8 is a valid CAS Registry Number.
InChI:InChI=1/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
109244-58-8Relevant articles and documents
Synthesis and Biological Studies of Iodinated (127/125I) Derivatives of Rhodamine 123
Kinsey, Berma M.,Kassis, Amin I.,Fayad, Fahed,Layne, Warren W.,Adelstein, S. James
, p. 1757 - 1761 (1987)
Rhodamine 123, a mitochondrial stain that preferentially accumulates in certain cancer cells, has been reduced and iodinated by using NaI in the presence of N-chlorosuccinimide.The various mono-, di-, and triiodo derivatives have been isolated and charact