17363-90-5Relevant articles and documents
Structure and function of bile. I. Relation between the structure of artificial bile and activity of pancreatic lipase
Uematsu,Unno,Niikura,Sawaumi,Akahori
, p. 3327 - 3336 (1984)
An aqueous solution of lecithin, bile salts, and cholesterol (artificial bile) which resembles human bile was studied by means of polarizing microscopy, electron microscopy, spin-probe electron spin resonance (ESR) spectroscopy, and pancreatic lipase treatment to digest fatty esters. The relation between the structure of artificial bile and the digestion of fatty esters by pancreatic lipase was investigated, and the following results were obtained. Artificial bile consists of bile salt micelles and bile salt-lecithin-cholesterol mixed micelles. Fatty esters are solubilized in these micelles. Lipase hydrolyzes fatty esters solubilized in bile salt micelles but not in bile salt-lecithin-cholesterol mixed micelles. Ranging from 0.1 to 0.4 μ in size, the macromolecular complex of bile salt-lecithin-cholesterol can be observed by electron microscopy.
Stabilization of polynucleotide complexes
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Page 5, (2008/06/13)
Polynucleotide complexes are stabilized by adding a cryoprotectant compound and lyophilizing the resulting formulation. The lyophilized formulations are milled or sieved into a dry powder formulation which may be used to deliver the polynucleotide complex. Delivery of the polynucleotide to a desired cell tissue is accomplished by contacting the tissue with the powder to rehydrate it. In a preferred embodiment, a dry powder formulation is used to induce genetic modification of a patient's lung tissue.