27025-41-8Relevant articles and documents
Interaction between Glutathione and Resveratrol in the Presence of Hydrogen Peroxide: A Kinetic Model
Zinatullina,Kasaikina,Khrameeva,Indeykina,Kononikhin
, p. 255 - 263 (2021/04/26)
Abstract: The kinetics of interaction between glutathione (GSH) and unsaturated phenol resveratrol (RVT) in deionized water in the presence of hydrogen peroxide (H2O2) is studied. At a physiological concentration (0.1–10 mM), GSH containing two carboxyl groups forms acidic solutions (pH of 3–4); the GSH molecules are associated into dimers. Under these conditions, GSH is quite slowly oxidized by atmospheric oxygen, and the reaction between GSH and H2O2 is accompanied by the formation of radicals. The thiyl radical initiation rate (Wi) is a few fractions of a percent of the GSH consumption rate; however, it is sufficient to initiate a thiol–ene chain reaction between GSH and RVT. Using the experimental data on the kinetics and the product composition and the published data on reactions of GSH with H2O2 and thiyl radicals, a kinetic model of the complex interaction between GSH and RVT in the presence of H2O2 in an aqueous medium at 37°C is proposed. The model includes 19 quasi-elementary reactions with respective rate constants, in particular, the formation of intermediate GSH–H2O2 and GSH–GSH complexes, the formation of radicals, and their subsequent transformations into final products in reactions with RVT and GSH. A computer simulation based on the developed model adequately describes the features of the process kinetics in a wide reactant concentration range.
Growth Factor-Decorated Ti3C2 MXene/MoS2 2D Bio-Heterojunctions with Quad-Channel Photonic Disinfection for Effective Regeneration of Bacteria-Invaded Cutaneous Tissue
Yang, Zhaopu,Fu, Xinliang,Ma, Daichuan,Wang, Yulin,Peng, Liming,Shi, Jiacheng,Sun, Jiyu,Gan, Xueqi,Deng, Yi,Yang, Weizhong
, (2021/11/01)
Phototherapy has recently emerged as a competent alternative for combating bacterial infection without antibiotic-resistance risk. However, owing to the bacterial endogenous antioxidative glutathione (GSH), the exogenous reactive oxygen species (ROS) generated by phototherapy can hardly behave desired antibacterial effect. To address the daunting issue, a quad-channel synergistic antibacterial nano-platform of Ti3C2 MXene/MoS2 (MM) 2D bio-heterojunctions (2D bio-HJs) are devised and fabricated, which possess photothermal, photodynamic, peroxidase-like (POD-like), and glutathione oxidase-like properties. Under near-infrared (NIR) laser exposure, the 2D bio-HJs both yield localized heating and raise extracellular ROS level, leading to bacterial inactivation. Synchronously, Mo4+ ions can easily invade into ruptured bacterial membrane, arouse intracellular ROS, and deplete intracellular GSH. Squeezed between the “ROS hurricane” from both internal and external sides, the bacteria are hugely slaughtered. After being further loaded with fibroblast growth factor-21 (FGF21), the 2D bio-HJs exhibit benign cytocompatibility and boost cell migration in vitro. Notably, the in vivo evaluations employing a mouse-infected wound model demonstrate the excellent photonic disinfection towards bacterial infection and accelerated wound healing. Overall, this work provides a powerful nano-platform for the effective regeneration of bacteria-invaded cutaneous tissue using 2D bio-HJs.
Alternative mechanisms of action for the apoptotic activity of terpenoid-like chalcone derivatives
Bernardes, Aline,Custodio, Jean M. F.,Molnár, Szilárd,Moura, Andrea F.,Noda-Perez, Caridad,Oliver, Allen G.,Perjési, Pál,Vaz, Wesley F.
supporting information, p. 15267 - 15279 (2021/09/07)
Apoptosis is a defense mechanism against pre-cancerous and infected cells. Although the applicability of β-ionone against diverse cancer cell lines has been exhaustively investigated, the apoptotic activity of terpenoid-like chalcones, as well as their mechanisms of action, is not well understood. Here we present a new terpenoid-like chalcone derivative (I) and its biological potential against HL-60 (leukemia), HCT-116 (colon), and SNB-19 (glioblastoma) cancer cell lines. CompoundIshowed cytotoxicity against over 90% of the tested cell lines. However,Ihas an IC50slightly higher than doxorubicin, a DNA-binding cancer drug, which motivated us to investigate an alternative mechanism of action forIother than DNA-mediated. We performed anin silicostructure-based pharmacophoric screening against various proteins, which indicated mitogen-activated protein kinase 5 (MAP3K5) as a potential protein target. CompoundIwas docked within its active site and was predicted to bind MAP3K5 with a comparable affinity to IM6, a cocrystallized ligand. Finally, we describe the reaction of a reduced glutathione adduct (GSH) with compoundIand previously published derivatives bearing the substitutions 4-chloro (II), 4-bromo (III), and 4-nitro (IV) using HPLC-MS. We show that these are rapid reactions and that the products are stable for up to 24 h. Here, we suggest two alternative mechanisms (MAP3K5 inhibition and thiol reactivity) for the biological potential of a series of terpenoid-like chalcone derivatives. We anticipate that these findings can be explored to design additional derivatives with even more robust apoptotic activity.
